Umadome H, Uchiyama T, Onishi R, Hori T, Uchino H, Nesumi N
First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Blood. 1988 Oct;72(4):1177-81.
Recently, interleukin-4 (IL-4) has been clarified as having T-cell growth factor activity; therefore, it becomes of interest whether IL-4, as well as interleukin-2 (IL-2), affects the proliferation of leukemic cells derived from mature T cells. In the present study, we describe a case of chronic T-lymphocytic leukemia (T-CLL) with monoclonal proliferation of human T-lymphotropic retrovirus (HTLV)-I or HTLV-II negative CD3(+)4(+)8(-) T cell expressing IL-2 receptors without stimulation. Radiolabeled IL-2 binding assay revealed 750 high-affinity and 6,750 low-affinity binding sites per cell. In accordance with the expression of high-affinity IL-2 receptors, the leukemic cells proliferated in response to exogenous IL-2 without prior stimulation. In addition, exogenous IL-4 also induced their proliferation. Moreover, IL-2 and IL-4 exerted a synergistic effect on the leukemic cell proliferation. Although the expression of IL-2 or IL-4 mRNA was not detected in fresh leukemic cells, the expression of IL-2 mRNA, but not IL-4 mRNA, was induced by phytohemagglutinin stimulation, and the leukemic cells proliferated. These findings suggest that not only IL-2, but also IL-4 are involved in the proliferation of leukemic cells of T-CLL.
最近，白细胞介素-4（IL-4）已被明确具有T细胞生长因子活性；因此，IL-4以及白细胞介素-2（IL-2）是否影响源自成熟T细胞的白血病细胞增殖变得令人关注。在本研究中，我们描述了一例慢性T淋巴细胞白血病（T-CLL），其人类嗜T淋巴细胞逆转录病毒（HTLV）-I或HTLV-II阴性的CD3(+)4(+)8(-) T细胞呈单克隆增殖，且无需刺激即可表达IL-2受体。放射性标记的IL-2结合试验显示每个细胞有750个高亲和力和6750个低亲和力结合位点。与高亲和力IL-2受体的表达一致，白血病细胞在无预先刺激的情况下对外源性IL-2产生增殖反应。此外，外源性IL-4也诱导其增殖。而且，IL-2和IL-4对白血病细胞增殖发挥协同作用。虽然在新鲜白血病细胞中未检测到IL-2或IL-4 mRNA的表达，但经植物血凝素刺激可诱导IL-2 mRNA而非IL-4 mRNA的表达，且白血病细胞发生增殖。这些发现表明，不仅IL-2，而且IL-4也参与T-CLL白血病细胞的增殖。
