Zhang Wei, Liu Hong, Li Xia, Liu Duo, Dong Xiu-Tao, Li Fei-Fei, Wang En-Xu, Li Bing-Zhi, Yuan Ying-Jin
Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology Tianjin University Tianjin P.R. China.
SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Tianjin University Tianjin P.R. China.
Eng Life Sci. 2017 Jun 1;17(9):1021-1029. doi: 10.1002/elsc.201700039. eCollection 2017 Sep.
Heterologous production of naringenin, a valuable flavonoid with various biotechnological applications, was well studied in the model organisms such as or . In this study, a synergistic co-culture system was developed for the production of naringenin from xylose by engineering microorganism. A long metabolic pathway was reconstructed in the co-culture system by metabolic engineering. In addition, the critical gene of 4-coumaroyl-CoA ligase () was simultaneously integrated into the yeast genome as well as a multi-copy free plasmid for increasing enzyme activity. On this basis, some factors related with fermentation process were considered in this study, including fermented medium, inoculation size and the inoculation ratio of two microbes. A yield of 21.16 ± 0.41 mg/L naringenin was produced in this optimized co-culture system, which was nearly eight fold to that of the mono-culture of yeast. This is the first time for the biosynthesis of naringenin in the co-culture system of and from xylose, which lays a foundation for future study on production of flavonoid.
柚皮素是一种具有多种生物技术应用价值的类黄酮，其在诸如[具体生物1]或[具体生物2]等模式生物中的异源生产已得到充分研究。在本研究中，通过对微生物进行工程改造，开发了一种用于从木糖生产柚皮素的协同共培养系统。通过代谢工程在共培养系统中重建了一条长代谢途径。此外，4-香豆酰辅酶A连接酶（[具体基因名称]）的关键基因被同时整合到酵母基因组以及多拷贝游离质粒中，以提高酶活性。在此基础上，本研究考虑了与发酵过程相关的一些因素，包括发酵培养基、接种量和两种微生物的接种比例。在这个优化的共培养系统中，柚皮素的产量为21.16±0.41mg/L，几乎是酵母单培养产量的八倍。这是首次在[两种生物名称]的共培养系统中从木糖生物合成柚皮素，为未来类黄酮生产的研究奠定了基础。
