Zhou Yiyao, Li Zhenghong, Wang Xiaonan, Zhang Haoran
Department of Chemical and Biochemical Engineering Rutgers the State University of New Jersey Piscataway NJ USA.
Eng Life Sci. 2019 Apr 2;19(5):389-395. doi: 10.1002/elsc.201800195. eCollection 2019 May.
Converting renewable feedstocks to aromatic compounds using engineered microbes offers a robust approach for sustainable, environment-friendly, and cost-effective production of these value-added products without the reliance on petroleum. In this study, rationally designed co-culture systems were established for converting glycerol to 3-hydroxybenzoic acid (3HB). Specifically, the 3HB pathway was modularized and accommodated by two metabolically engineered strains. The co-culture biosynthesis was optimized by using different cultivation temperatures, varying the inoculum ratio between the co-culture strains, recruitment of a key pathway intermediate transporter, strengthening the critical pathway enzyme expression, and adjusting the timing for inducing pathway gene expression. Compared with the mono-culture, the optimized co-culture showed 5.3-fold improvement for 3HB biosynthesis. This study demonstrated the applicability of modular co-culture engineering for addressing the challenges of aromatic compound biosynthesis.
利用工程微生物将可再生原料转化为芳香族化合物，为可持续、环保且经济高效地生产这些增值产品提供了一种可靠的方法，且无需依赖石油。在本研究中，构建了经过合理设计的共培养系统，用于将甘油转化为3-羟基苯甲酸（3HB）。具体而言，3HB途径被模块化，并由两种代谢工程菌株来实现。通过采用不同的培养温度、改变共培养菌株之间的接种比例、引入关键途径中间产物转运体、增强关键途径酶的表达以及调整诱导途径基因表达的时间，对共培养生物合成进行了优化。与单培养相比，优化后的共培养在3HB生物合成方面提高了5.3倍。本研究证明了模块化共培养工程在应对芳香族化合物生物合成挑战方面的适用性。
