Thuan Nguyen Huy, Tatipamula Vinay Bharadwaj, Canh Nguyen Xuan, Van Giang Nguyen
Center for Molecular Biology, Duy Tan University, Da Nang, 550000 Vietnam.
Faculty of Biotechnology, Vietnam National University of Agriculture, Gialam, Hanoi Vietnam.
3 Biotech. 2022 May;12(5):115. doi: 10.1007/s13205-022-03177-4. Epub 2022 Apr 19.
Micro-organisms have often been used to produce bioactive compounds as antibiotics, antifungals, and anti-tumors, etc. due to their easy and applicable culture, genetic manipulation, and extraction, etc. Mainly, microbial mono-cultures have been applied to produce value-added compounds and gotten numerous valuable results. However, mono-culture also has several complicated problems, such as metabolic burdens affecting the growth and development of the host, leading to a decrease in titer of the target compound. To circumvent those limitations, microbial co-culture has been technically developed and gained much interest compared to mono-culture. For example, co-culture simplifies the design of artificial biosynthetic pathways and restricts the recombinant host's metabolic burden, causing increased titer of desired compounds. This paper summarizes the recent advanced progress in applying microbial platform co-culture to produce natural products, such as flavonoid, terpenoid, alkaloid, etc. Furthermore, importantly different strategies for enhancing production, overcoming the metabolic burdens, building autonomous modulation of cell growth rate and culture composition in response to a quorum-sensing signal, etc., were also described in detail.
由于微生物易于培养、便于进行基因操作和提取等，它们常被用于生产抗生素、抗真菌剂和抗肿瘤药物等生物活性化合物。主要是，微生物单培养已被用于生产增值化合物并取得了许多有价值的成果。然而，单培养也存在一些复杂问题，例如代谢负担会影响宿主的生长和发育，导致目标化合物的产量下降。为了克服这些限制，与单培养相比，微生物共培养技术得到了发展并引起了广泛关注。例如，共培养简化了人工生物合成途径的设计，并限制了重组宿主的代谢负担，从而提高了所需化合物的产量。本文总结了应用微生物平台共培养生产天然产物（如黄酮类、萜类、生物碱等）的最新研究进展。此外，还详细描述了提高产量、克服代谢负担、建立响应群体感应信号的细胞生长速率和培养组成的自主调节等重要的不同策略。
