Department of Oncology, Huzhou Cent Hosp, Affiliated Cent Hosp Huzhou University, 198 Hongqi Rd, Huzhou, Zhejiang, China.
Department of Oncology, Huzhou Central Hospital, 198 Hongqi Road, Huzhou, Zhejiang Province 313000, China.
Dis Markers. 2020 Jun 16;2020:9369341. doi: 10.1155/2020/9369341. eCollection 2020.
Thyroid carcinoma (THCA) is one of the most common malignancies of the endocrine system, which is usually treated by surgery combined with iodine-131 (I) radiotherapy.
This study is aimed at exploring the potential targets of I radiotherapy in THCA.
The RNA-sequencing data of THCA in The Cancer Genome Atlas database (including 568 THCA samples) was downloaded. The differentially expressed genes (DEGs) between the tumour samples whether or not subjected to I radiotherapy were identified using edgeR package. Using the WGCNA package, the module that was relevant with I radiotherapy was selected. The intersection genes of the hub module nodes and the DEGs were obtained as hub genes, followed by the function and pathway enrichment analyses using the clusterProfiler package. Moreover, the protein-protein interaction (PPI) network for the hub genes was constructed using Cytoscape software. In addition, more important hub genes were analysed with function mining using the GenCLiP2 online tool. The qPCR analysis was used to verify the mRNA expression of more important hub genes in THCA tissues.
There were 500 DEGs (167 upregulated and 333 downregulated) between the two groups. WGCNA analysis showed that the green module (428 nodes) exhibited the most significant correlation with I radiotherapy. A PPI network was built after the identification of 53 hub genes. In the PPI network, , , , , , , , , and exhibited higher degrees, which were mainly implicated in the vascular function. The relative expression of nine mRNAs in the THCA tissues treated with I was lower.
I radiotherapy might exert therapeutic effects by targeting , , , , , , , , and in THCA patients.
甲状腺癌（THCA）是内分泌系统最常见的恶性肿瘤之一，通常采用手术联合碘-131（I）放疗进行治疗。
本研究旨在探讨 I 放疗在 THCA 中的潜在靶点。
从癌症基因组图谱（TCGA）数据库中下载 THCA 的 RNA-seq 数据（包含 568 例 THCA 样本）。使用 edgeR 包鉴定接受或未接受 I 放疗的肿瘤样本之间的差异表达基因（DEGs）。使用 WGCNA 包，选择与 I 放疗相关的模块。获得 hub 模块节点与 DEGs 的交集基因作为 hub 基因，然后使用 clusterProfiler 包进行功能和通路富集分析。此外，使用 Cytoscape 软件构建 hub 基因的蛋白质-蛋白质相互作用（PPI）网络。此外，使用 GenCLiP2 在线工具对更重要的 hub 基因进行功能挖掘分析。采用 qPCR 分析验证 THCA 组织中更重要的 hub 基因的 mRNA 表达。
两组间有 500 个 DEGs（167 个上调，333 个下调）。WGCNA 分析显示，绿色模块（428 个节点）与 I 放疗相关性最强。鉴定出 53 个 hub 基因后构建了 PPI 网络。在 PPI 网络中，、、、、、、、、和 具有较高的度数，主要涉及血管功能。经 I 治疗的 THCA 组织中 9 个 mRNA 的相对表达水平较低。
I 放疗可能通过靶向 THCA 患者中的、、、、、、、和 发挥治疗作用。
