Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Biomed Res Int. 2020 Jun 14;2020:3765937. doi: 10.1155/2020/3765937. eCollection 2020.
Single nucleotide polymorphism (SNP) of complement component 2 (C2) has been found to be significantly associated with hepatocellular carcinoma (HCC). However, little is known about the role and mechanism of C2 in HCC. In the present study, we aimed to explore the prognostic value of C2 and its correlation with tumor-infiltrating immune cells in HCC.
mRNA expression was downloaded from TCGA (365 HCC patients and 50 healthy controls), GSE14520 (220 HCC patients and 220 adjacent normal tissues), and ICGC HCC (232 HCC patients) cohorts. Unpaired Student's -tests or ANOVA tests were used to evaluate differences of C2 expression. Univariate and multivariate analyses were used to analyze the prognostic value of C2. CIBERSORT was used to calculate the proportion of 22 kinds of tumor-infiltrating immune cells.
Significantly lower C2 expression was found at HCC compared to healthy controls, and C2 was associated with TNM stages. Higher C2 expression was significantly associated with better prognosis, and multivariate analysis showed that C2 was also an independent factor for the prognosis of HCC. Moreover, elevated CD4 T cells were found at HCC patients with higher C2 expression while the higher proportion of macrophage M0 cells was found in HCC patients with lower C2 expression. KEGG analysis showed that "cell cycle," "AMPK signaling pathway," and "PPAR signaling pathway" were enriched in HCC patients with higher C2 expression.
C2 is a prognostic factor for HCC and may be used as a therapeutic target for future treatment of HCC.
补体成分 2（C2）的单核苷酸多态性（SNP）已被发现与肝细胞癌（HCC）显著相关。然而，关于 C2 在 HCC 中的作用和机制知之甚少。在本研究中，我们旨在探讨 C2 的预后价值及其与 HCC 肿瘤浸润免疫细胞的相关性。
从 TCGA（365 例 HCC 患者和 50 例健康对照）、GSE14520（220 例 HCC 患者和 220 例相邻正常组织）和 ICGC HCC（232 例 HCC 患者）队列中下载 mRNA 表达数据。使用非配对学生 t 检验或 ANOVA 检验评估 C2 表达的差异。单因素和多因素分析用于分析 C2 的预后价值。CIBERSORT 用于计算 22 种肿瘤浸润免疫细胞的比例。
与健康对照组相比，HCC 中 C2 的表达明显降低，并且 C2 与 TNM 分期相关。较高的 C2 表达与较好的预后显著相关，多因素分析表明 C2 也是 HCC 预后的独立因素。此外，在 C2 表达较高的 HCC 患者中，CD4 T 细胞比例升高，而在 C2 表达较低的 HCC 患者中，M0 巨噬细胞的比例较高。KEGG 分析表明，在 C2 表达较高的 HCC 患者中，“细胞周期”、“AMPK 信号通路”和“PPAR 信号通路”富集。
C2 是 HCC 的预后因素，可能作为未来 HCC 治疗的治疗靶点。
