Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur 3-5, 400372, Cluj-Napoca, Romania.
Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776, Warsaw, Poland.
Pol J Vet Sci. 2020 Jun;23(2):229-233. doi: 10.24425/pjvs.2020.133637.
A disease survey was conducted between 2014 and 2018 in the Polish goat population to determine the seroprevalence of pestiviral infection. Blood samples from 910 goats (782 females and 128 males) were collected in 62 goat herds and tested for bovine viral diarrhea virus (BVDV) infection with a competitive ELISA in a serial fashion. Between 10 and 13 adult female goats were randomly selected from each herd, assuming individual-level seroprevalence of pestiviral infection in a herd of at least 30% and a level of confidence of 95%. In each herd, all males were tested. At least one seropositive goat was found in each of the 4 herds. However, in one herd, the only positive goat tested negative in serial retesting. Finally, 3 herds were consi-dered as seropositive which yielded apparent herd-level seroprevalence of 4.8% (CI 95%: 1.7%, 13.3%). After adjusting for the ELISA herd-level sensitivity and specificity, the true herd-level prevalence was 3.9% (CI 95%: 1.2%, 12.0%). No males tested positive. In 2 out of 3 seropositive herds, goats regularly shared pastures with cattle.
2014 年至 2018 年，在波兰山羊群体中进行了一次疾病调查，以确定流行病毒感染的血清流行率。从 62 个山羊群中采集了 910 只山羊（782 只雌性和 128 只雄性）的血液样本，并采用连续式竞争 ELISA 检测牛病毒性腹泻病毒（BVDV）感染。每个羊群中，随机选择 10 到 13 只成年雌性山羊，假设每个羊群中流行病毒感染的个体血清流行率至少为 30%，置信水平为 95%。在每个羊群中，所有雄性都接受了检测。在 4 个羊群中，每个羊群都发现了至少一只血清阳性山羊。然而，在一个羊群中，唯一的阳性山羊在连续重复检测中呈阴性。最终，3 个羊群被认为是血清阳性的，其血清流行率为 4.8%（95%CI：1.7%，13.3%）。在调整 ELISA 群体水平敏感性和特异性后，真实的群体水平流行率为 3.9%（95%CI：1.2%，12.0%）。没有雄性检测呈阳性。在 3 个血清阳性的羊群中，有 2 个羊群的山羊定期与牛共享牧场。
