State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products / Key Laboratory for Pesticide Residue Detection of Ministry of Agriculture / Laboratory (Hangzhou) for Risk Assessment of Agricultural Products of Ministry of Agriculture, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, Zhejiang, China.
College of Environment, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, China.
Environ Pollut. 2020 Nov;266(Pt 3):115067. doi: 10.1016/j.envpol.2020.115067. Epub 2020 Jun 22.
Aquatic organisms are usually exposed to various co-existing pollutants. However, toxic effects of pesticide mixtures on aquatic organisms and its potential underlying mechanism still remain unclear. The joint effects of fenpropathrin (FEN) and paclobutrazol (PAC) on zebrafish (Danio rerio) using diverse toxicological endpoints were investigated in the current work. Our data exhibited that the 96-h LC values of FEN to zebrafish at multiple life phases ranged from 0.0029 (0.0013-0.0042) to 0.16 (0.082-0.23) mg a.i. L, which were lower by comparison to PAC ranging from 13.16 (8.564-21.03) to 23.43 (17.94-29.91) mg a.i. L. Combination of FEN and PAC displayed synergistic effect on embryonic zebrafish. Activities of T-SOD, Cu/Zn-SOD and CYP450 were remarkably changed in the majority of single and mixture treatments by comparison to the untreated group. The mRNA levels of 17 genes related to oxidative stress, cellular apoptosis, immune system and endocrine system were assessed, and the data suggested that embryonic zebrafish were affected by both single pesticides and their mixtures. Five genes (P53, tsh, ERα, crh and cxcl-clc) showed greater alterations when exposed to pesticide mixtures by comparison to their individual chemicals. Therefore, it is urgently necessary to conduct more studies on mixture toxicities of different pesticides to explore the chemical mixtures with synergistic interactions.
水生生物通常会接触到各种共存的污染物。然而，农药混合物对水生生物的毒性作用及其潜在的机制仍不清楚。本研究采用多种毒理学终点，研究了高效氯氟氰菊酯（FEN）和多效唑（PAC）混合物对斑马鱼（Danio rerio）的联合作用。我们的数据表明，FEN 对斑马鱼多个生命阶段的 96 h LC 值范围为 0.0029（0.0013-0.0042）至 0.16（0.082-0.23）mg a.i. L，与 PAC 的 13.16（8.564-21.03）至 23.43（17.94-29.91）mg a.i. L 相比有所降低。FEN 和 PAC 的组合对胚胎斑马鱼表现出协同作用。与未处理组相比，大多数单一和混合物处理组的 T-SOD、Cu/Zn-SOD 和 CYP450 活性均显著改变。评估了与氧化应激、细胞凋亡、免疫系统和内分泌系统相关的 17 个基因的 mRNA 水平，数据表明，单一农药及其混合物均影响胚胎斑马鱼。与单一化学物质相比，当暴露于农药混合物时，有 5 个基因（P53、tsh、ERα、crh 和 cxcl-clc）表现出更大的变化。因此，迫切需要开展更多不同农药混合物毒性的研究，以探索具有协同作用的化学混合物。
