Khoo Jing Jing, Kurtti Timothy J, Husin Nurul Aini, Beliavskaia Alexandra, Lim Fang Shiang, Zulkifli Mulya Mustika Sari, Al-Khafaji Alaa M, Hartley Catherine, Darby Alistair C, Hughes Grant L, AbuBakar Sazaly, Makepeace Benjamin L, Bell-Sakyi Lesley
Tropical Infectious Diseases Research and Education Centre, Level 2, High Impact Research Building, University of Malaya, Kuala Lumpur 50603, Malaysia.
Department of Entomology, University of Minnesota, 219 Hodson Hall, 1980 Folwell Avenue, Saint Paul, MN 55108, USA.
Microorganisms. 2020 Jul 1;8(7):988. doi: 10.3390/microorganisms8070988.
are intracellular endosymbionts of several invertebrate taxa, including insects and nematodes. Although DNA has been detected in ticks, its presence is generally associated with parasitism by insects. To determine whether or not can infect and grow in tick cells, cell lines from three tick species, , and , were inoculated with strains Stri and AlbB isolated from mosquito cell lines. Homogenates prepared from fleas collected from cats in Malaysia were inoculated into an cell line. Bacterial growth and identity were monitored by microscopy and PCR amplification and sequencing of fragments of genes. The Stri strain infected spp. cells and was maintained through 29 passages. The AlbB strain successfully infected spp. and cells and was maintained through 2-5 passages. A novel strain of belonging to the supergroup F, designated CfeF, was isolated in cells from a pool of sp. cat fleas and maintained in vitro through two passages over nine months. This is the first confirmed isolation of a strain from a flea and the first isolation of any strain outside the "pandemic" A and B supergroups. The study demonstrates that tick cells can host multiple strains, and can be added to panels of insect cell lines to improve success rates in isolation of field strains of .
是几种无脊椎动物类群（包括昆虫和线虫）的细胞内共生体。虽然在蜱中已检测到DNA，但其存在通常与昆虫寄生有关。为了确定是否能在蜱细胞中感染和生长，将从蚊子细胞系中分离出的菌株Stri和AlbB接种到三种蜱种（、和）的细胞系中。将从马来西亚猫身上采集的跳蚤制备的匀浆接种到细胞系中。通过显微镜检查以及对基因片段进行PCR扩增和测序来监测细菌的生长和鉴定。Stri菌株感染了 spp.细胞，并传代29次。AlbB菌株成功感染了 spp.和细胞，并传代2 - 5次。在来自 sp.猫蚤群体的细胞中分离出一种属于超群F的新型菌株，命名为CfeF，并在体外传代两次，历时九个月。这是首次从跳蚤中确认分离出菌株，也是首次在“大流行”的A和B超群之外分离出任何菌株。该研究表明蜱细胞可以容纳多种菌株，并且可以添加到昆虫细胞系组中以提高分离野外菌株的成功率。
