Matos Rita, De Witte Chloë, Smet Annemieke, Berlamont Helena, De Bruyckere Sofie, Amorim Irina, Gärtner Fátima, Haesebrouck Freddy
Instituto de Investigação e Inovação em Saúde da Universidade do Porto (i3S), 4200-135 Porto, Portugal.
Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal.
Microorganisms. 2020 Jun 25;8(6):957. doi: 10.3390/microorganisms8060957.
A combined agar and broth dilution method followed by qPCR was used to determine the antimicrobial susceptibility of feline and isolates. All isolates showed a monomodal distribution of MICs for all the antimicrobial agents tested. For , a bimodal distribution was observed for azithromycin, enrofloxacin, spectinomycin, and lincomycin. Single nucleotide polymorphisms (SNPs) were found in 50S ribosomal proteins L2 and L3 of the isolate not belonging to the WT population for azithromycin, and in 30S ribosomal proteins S1, S7, and S12 of the isolate not belonging to the WT population for spectinomycin. The antimicrobial resistance mechanism to enrofloxacin and lincomycin remains unknown (2 and 1 isolate(s), resp.). Furthermore, isolates showed higher MICs for neomycin compared to isolates which may be related to the presence of SNPs in several 30S and 50S ribosomal protein encoding genes and ribosomal RNA methyltransferase genes. This study shows that acquired resistance to azithromycin, spectinomycin, enrofloxacin, and lincomycin occasionally occurs in feline isolates. As pets may constitute a source of infection for humans, this should be kept in mind when dealing with a human patient infected with .
采用琼脂和肉汤稀释联合方法并结合定量聚合酶链反应（qPCR）来测定猫源分离株的抗菌药敏性。所有分离株对所有测试抗菌药物的最低抑菌浓度（MIC）均呈现单峰分布。对于[具体菌名未明确]，观察到阿奇霉素、恩诺沙星、壮观霉素和林可霉素的MIC呈双峰分布。在不属于野生型群体的[具体菌名未明确]分离株中，发现阿奇霉素相关的50S核糖体蛋白L2和L3存在单核苷酸多态性（SNP），在不属于野生型群体的[具体菌名未明确]分离株中，发现壮观霉素相关的30S核糖体蛋白S1、S7和S12存在SNP。对恩诺沙星和林可霉素的耐药机制仍不清楚（分别为2株和1株分离株）。此外，[具体菌名未明确]分离株对新霉素的MIC高于[另一具体菌名未明确]分离株，这可能与多个30S和50S核糖体蛋白编码基因以及核糖体RNA甲基转移酶基因中存在SNP有关。本研究表明，猫源[具体菌名未明确]分离株偶尔会出现对阿奇霉素、壮观霉素、恩诺沙星和林可霉素的获得性耐药。由于宠物可能是人类感染源，在处理感染[具体菌名未明确]的人类患者时应牢记这一点。
