Tay Andy
Department of Biomedical Engineering, National University of Singapore, Singapore 117583.
ACS Nano. 2020 Jul 28;14(7):7714-7721. doi: 10.1021/acsnano.0c04624. Epub 2020 Jul 6.
Nanostructures, with their localized interactions with mammalian cells, can offer better efficiency and lower cell perturbation than conventional viral, biochemical, and electroporation transfection techniques. In this Perspective, I describe the different stages of transfection and provide a comparison of transfection techniques based on their mechanisms. Focusing on specific aims of transfection, I also illustrate how recent developments in high-aspect-ratio nanostructures have endowed them with properties that are superior to existing viral, biochemical, and electroporation methods as a versatile technique to deliver a variety of cargoes and to interface with different mammalian cell types for biomedical applications. Finally, I describe the challenges associated with transfection that need to be overcome to enhance cargo delivery efficiency and clinical translation.
纳米结构与哺乳动物细胞的局部相互作用，相比传统的病毒、生化和电穿孔转染技术，能提供更高的效率和更低的细胞扰动。在这篇观点文章中，我描述了转染的不同阶段，并基于其机制对转染技术进行了比较。聚焦于转染的特定目标，我还阐述了高纵横比纳米结构的最新进展如何使其具备优于现有病毒、生化和电穿孔方法的特性，成为一种通用技术，可用于递送各种货物并与不同类型的哺乳动物细胞相互作用以用于生物医学应用。最后，我描述了与转染相关的挑战，这些挑战需要被克服以提高货物递送效率和临床转化。
