Gallic acid mitigates diclofenac-induced liver toxicity by modulating oxidative stress and suppressing gene expression in male rats.

CONTEXT

Diclofenac (DIC) is an NSAID and consumption of this drug creates side effects such as liver injury. Gallic acid (GA), a natural component of many plants, is used as an antioxidant agent.

OBJECTIVE

This study assesses the hepatoprotective effects of GA in the rat model of DIC-induced liver toxicity.

MATERIALS AND METHODS

In this research, the male Wistar rats were separated into five groups ( = 6). Group 1, control, received normal saline (1 mL/kg bw, i.p.); Group 2 received DIC-only (50 mg/kg bw, i.p.); Groups 3, received DIC (50 mg/kg bw, i.p.) plus silymarin (100 mg/kg bw, po), groups 4 and 5 received DIC (50 mg/kg bw, i.p.) plus GA (50 and 100 mg/kg, po, respectively).

RESULTS

The data demonstrated that the liver levels of the GSH, GPx, SOD, and CAT significantly reduced and the levels of the serum protein carbonyl, AST, ALP, ALT, total bilirubin, MDA, serum IL-1β, and the liver gene expression were remarkably increased in the second group compared to control group. On the other hand, treatment with GA led to a significant elevation in GSH, GPx, SOD, CAT, and a significant decrease in protein carbonyl, AST, ALP, ALT, total bilirubin, MDA, serum IL-1β, and gene expression of in comparison with the second group. Histological changes were also ameliorated by GA oral administration. The data show that the oral administration of GA could alleviate the noxious effects of DIC on the antioxidant defense system and liver tissue.