El-Sherbiny Gamal M, Alluqmani Amira J, Elsehemy Islam A, Kalaba Mohamed H
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
Biology Department, Umm Al-Qura University, 21421, Makkah, Saudi Arabia.
Sci Rep. 2024 Dec 16;14(1):30485. doi: 10.1038/s41598-024-80700-y.
Bacterial resistance to antibiotics remains a significant clinical challenge, contributing to persistently high rates of morbidity and mortality. Achieving treatment success is increasingly difficult, necessitating the evaluation of new antibiotics and complementary approaches, including source control and alternative therapies. This study aimed to investigate the antibacterial, antioxidant, cytotoxic, and phytochemical properties of Moringa oleifera leaf extract using high-performance liquid chromatography (HPLC), and to evaluate the pharmacokinetic properties of its major compound. The extract demonstrated strong antibacterial activity against standard strains and foodborne bacterial species. It also showed significant antioxidant potential, supported by the presence of high concentrations of phenolic and flavonoid compounds. HPLC analysis identified multiple bioactive compounds, with quercetin as the predominant component. The cytotoxicity study confirmed the safety of the extract at low and moderate concentrations, and ADMET analysis indicated favorable pharmacokinetic characteristics of quercetin. In conclusion, Moringa oleifera exhibits promising potential for medical and food industry applications due to its significant antibacterial and antioxidant activities, combined with a strong safety profile and rich phytochemical content.
细菌对抗生素的耐药性仍然是一个重大的临床挑战，导致发病率和死亡率持续居高不下。实现治疗成功越来越困难，因此需要评估新的抗生素和补充方法，包括源头控制和替代疗法。本研究旨在利用高效液相色谱法（HPLC）研究辣木叶提取物的抗菌、抗氧化、细胞毒性和植物化学性质，并评估其主要化合物的药代动力学性质。该提取物对标准菌株和食源细菌具有很强的抗菌活性。它还表现出显著的抗氧化潜力，这由高浓度的酚类和黄酮类化合物的存在所支持。HPLC分析鉴定出多种生物活性化合物，其中槲皮素是主要成分。细胞毒性研究证实了该提取物在低浓度和中等浓度下的安全性，ADMET分析表明槲皮素具有良好的药代动力学特性。总之，辣木因其显著的抗菌和抗氧化活性，以及强大的安全性和丰富的植物化学成分，在医疗和食品工业应用中具有广阔的前景。
