Department of Physiology, Iran University of Medical Sciences, Tehran, Iran.
Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Arch Med Res. 2021 May;52(4):380-388. doi: 10.1016/j.arcmed.2020.12.005. Epub 2020 Dec 23.
BACKGROUND/AIM: Diclofenac (DIC) is a Nonsteroidal anti-inflammatory drug (NSAID) and consumption of this drug creates side effects such as renal injury. The purpose of this work was to assess the influences of gallic acid (GA) on DIC-induced renal injury in rats.
Rats were segregated into five groups. Group 1, control group; Group 2 received DIC-only (50 mg/kg bw, i.p.) for 7 consecutive days; Groups 3, received GA-only (100 mg/kg bw, po) for 7 consecutive days; group 4 received DIC (50 mg/kg bw, i.p.) plus GA (50 mg/kg, po) for 7 consecutive days and group 5 received DIC (50 mg/kg bw, i.p.) plus GA (100 mg/kg, po) for 7 consecutive days.
The data indicated that the levels of the serum protein carbonyl, sGOT, sGPT, urea, creatinine, uric acid, nitrite content, MDA, serum IL-1β, and the renal IL-1β gene expression were remarkably increased in DIC-only treated animals compared to control group. In the other hand, treatment with gallic acid led to significant improvements in abnormalities of DIC-induced oxidative stress and serum biochemical parameters. Histological changes were also ameliorated by GA oral administration.
The results indicated that oral injection of GA could alleviate the noxious effects of DIC on the antioxidant defense system and renal tissue.
背景/目的：双氯芬酸（DIC）是一种非甾体抗炎药（NSAID），其使用会产生肾损伤等副作用。本工作旨在评估没食子酸（GA）对 DIC 诱导的大鼠肾损伤的影响。
将大鼠分为五组。第 1 组为对照组；第 2 组仅给予 DIC（50mg/kg bw，ip）连续 7 天；第 3 组仅给予 GA（100mg/kg bw，po）连续 7 天；第 4 组给予 DIC（50mg/kg bw，ip）加 GA（50mg/kg，po）连续 7 天；第 5 组给予 DIC（50mg/kg bw，ip）加 GA（100mg/kg，po）连续 7 天。
数据表明，与对照组相比，DIC 单独处理的动物血清蛋白羰基、sGOT、sGPT、尿素、肌酐、尿酸、亚硝酸盐含量、MDA、血清 IL-1β和肾 IL-1β 基因表达水平显著升高。另一方面，GA 治疗导致 DIC 诱导的氧化应激和血清生化参数异常显著改善。GA 口服给药还改善了组织学变化。
结果表明，GA 口服注射可减轻 DIC 对抗氧化防御系统和肾组织的有害影响。
