一种源自人尿的白细胞介素1抑制剂。与脱氧核糖核酸酶I同源。
A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I.
作者信息
Rosenstreich D L, Tu J H, Kinkade P R, Maurer-Fogy I, Kahn J, Barton R W, Farina P R
机构信息
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
出版信息
J Exp Med. 1988 Nov 1;168(5):1767-79. doi: 10.1084/jem.168.5.1767.
We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chromatography, hydrophobic affinity chromatography, hydroxylapatite chromatography, fast protein liquid chromatography, and two HPLC steps). SDS-PAGE analysis indicates that the purified material is a 38-kD molecule. Evidence based on a partial amino acid sequence analysis as well as enzyme studies indicates that this inhibitor is a type of human DNase I.
我们之前曾报道，发热患者的尿液中含有大量抑制白细胞介素-1诱导的小鼠胸腺细胞增殖的物质，该物质是一种大小在30至40kD之间的糖蛋白。在本研究中，通过一系列八个纯化步骤（硫酸铵沉淀、离子交换色谱、分子筛色谱、疏水亲和色谱、羟基磷灰石色谱、快速蛋白质液相色谱以及两个高效液相色谱步骤）将该因子纯化至同质。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，纯化后的物质是一个38-kD的分子。基于部分氨基酸序列分析以及酶学研究的证据表明，这种抑制剂是一种人脱氧核糖核酸酶I。
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