一种在体外影响淋巴细胞反应的人可溶性抑制因子。
A human soluble suppressor factor affecting lymphocyte responses in vitro.
作者信息
Shou L, Schwartz S A, Good R A, Peng R, Chen C L
出版信息
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6096-100. doi: 10.1073/pnas.77.10.6096.
A soluble suppressor factor (SSF) has been demonstrated in the supernatant of normal human peripheral blood lymphocyte cultures that exhibits suppressive activity toward the proliferative response of normal lymphocytes to concanavalin A or alloantigens in mixed lymphocyte culture (MLC) or toward pokeweed mitogen-stimulated immunoglobulin synthesis and secretion in vitro. Suppression of the proliferative response in MLC reached maximal levels when added SSF-containing supernatant approximated 20% by volume of the culture medium. Suppression in the MLC was found to act at the proliferative stage. SSF acts independently of cytotoxicity and is stable at 56 degrees C for 30 min but is inactivated at higher temperatures. Addition of SSF to the MLC as late as day 4 after initiation of the culture results in suppression of transformation. This factor(s) may regulate the magnitude of several immune responses in humans.
在正常人外周血淋巴细胞培养上清液中已证实存在一种可溶性抑制因子（SSF），它对正常淋巴细胞在混合淋巴细胞培养（MLC）中对刀豆球蛋白A或同种异体抗原的增殖反应，或对体外商陆有丝分裂原刺激的免疫球蛋白合成及分泌均表现出抑制活性。当添加含SSF的上清液占培养基体积约20%时，MLC中的增殖反应抑制达到最大水平。发现MLC中的抑制作用发生在增殖阶段。SSF的作用独立于细胞毒性，在56℃下30分钟稳定，但在更高温度下失活。在培养开始后第4天很晚时向MLC中添加SSF会导致转化受到抑制。这种因子可能调节人类多种免疫反应的强度。
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