Molecular Neurophysiology Research Group, Biomedical Research Institute, The National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki 305-8566, Japan.
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan.
Int J Mol Sci. 2020 Jul 5;21(13):4772. doi: 10.3390/ijms21134772.
Indonesian ginger ( Rosc.), also known as Bangle, exhibits neurotrophic effects on cultured murine cortical neurons and in the adult mouse brain, but the underlying mechanisms remain unknown. Here, using human fetal neural stem cells (hfNSCs) as a model system for in vitro human neurogenesis, we show that Bangle extracts activate canonical WNT/β-catenin signaling. Bangle extract-treatment of hfNSCs not only promoted neuronal differentiation, but also accelerated neurite outgrowth from immature neurons. Furthermore, Bangle extracts induced expression of neurogenic genes and WNT signaling-target genes, and facilitated the accumulation of β-catenin in nuclei of hfNSC. Interestingly, altered histone modifications were also observed in Bangle-treated hfNSCs. Together, these findings demonstrate that Bangle contributes to hfNSC neurogenesis by WNT pathway and epigenetic regulation.
印度尼西亚姜（ Rosc.），又名豆蔻，对培养的鼠皮质神经元和成年鼠脑具有神经营养作用，但作用机制尚不清楚。在这里，我们使用人胎神经干细胞（hfNSC）作为体外人类神经发生的模型系统，表明豆蔻提取物激活了经典的 WNT/β-catenin 信号通路。豆蔻提取物处理 hfNSC 不仅促进神经元分化，还加速未成熟神经元的轴突生长。此外，豆蔻提取物诱导神经发生基因和 WNT 信号靶基因的表达，并促进 hfNSC 中 β-catenin 向核内聚集。有趣的是，豆蔻处理的 hfNSC 中还观察到组蛋白修饰的改变。总之，这些发现表明豆蔻通过 WNT 通路和表观遗传调控促进 hfNSC 的神经发生。
