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组蛋白折叠着丝粒蛋白 W（CENP-W）与肝癌细胞的生物学行为有关。

Histone-fold centromere protein W (CENP-W) is associated with the biological behavior of hepatocellular carcinoma cells.

机构信息

Molecular Imaging Center, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University , Zhuhai, China.

Department of Experimental Medicine, The Fifth Affiliated Hospital, Sun Yat-sen University , Zhuhai, China.

出版信息

Bioengineered. 2020 Dec;11(1):729-742. doi: 10.1080/21655979.2020.1787776.

DOI:10.1080/21655979.2020.1787776
PMID:32635817
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8291794/
Abstract

Centromere protein W (CENP-W), identified as a centromeric component, plays an important role in the cell life cycle. However, how expression affects biological processes in liver cancer cells remains unknown. In this article, we found that was overexpressed in liver cancer tissues. Low expression was correlated with a better prognosis in hepatocellular carcinoma (HCC) patients, compared to high expression. The results of qRT-PCR and western blot assay showed that was effectively knocked down in HCC cells using siRNA transfection. Cell proliferation, migration, and invasion were inhibited. Cell apoptosis rates were increased. The cells were arrested in the G2/M phase of the cell cycle. Subsequently, 127 differentially expressed genes (DEGs) were identified based on RNA-seq data. GO and KEGG enrichment and PPI network analysis were performed. The novel DEGs were found and mainly enriched in nucleosome assembly and the complement system. In summary, our study indicated that overexpression of implied unfavorable prognosis and might be the potential predictive biomarker in liver cancer. Downregulation of might inhibit the HCC developmentby regulating the expression of the molecules in nucleosomes and the complement system.

摘要

着丝粒蛋白 W（CENP-W）作为一种着丝粒成分，在细胞生命周期中发挥着重要作用。然而，其表达如何影响肝癌细胞中的生物过程尚不清楚。在本文中，我们发现 CENP-W 在肝癌组织中过表达。与高表达相比，低表达与肝细胞癌（HCC）患者的较好预后相关。qRT-PCR 和 Western blot 检测结果表明，siRNA 转染可有效敲低 HCC 细胞中的 CENP-W。细胞增殖、迁移和侵袭受到抑制。细胞凋亡率增加。细胞周期被阻滞在 G2/M 期。随后，根据 RNA-seq 数据鉴定出 127 个差异表达基因（DEGs）。进行了 GO 和 KEGG 富集分析以及 PPI 网络分析。发现了新的 DEGs，主要富集在核小体组装和补体系统中。总之，我们的研究表明，CENP-W 的过表达提示预后不良，并且 CENP-W 可能是肝癌的潜在预测性生物标志物。下调 CENP-W 可能通过调节核小体和补体系统中分子的表达来抑制 HCC 的发展。

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