Li Qiang, He Xiaohui, Ren Yuanhang, Xiong Chuan, Jin Xin, Peng Lianxin, Huang Wenli
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, China.
Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
Front Microbiol. 2020 Jun 19;11:1382. doi: 10.3389/fmicb.2020.01382. eCollection 2020.
In this present study, we assembled and analyzed the mitogenomes of two asymbiotic and six ectomycorrhizal species based on next-generation sequencing data. The size of the eight mitogenomes ranged from 37,341 to 137,428 bp, and we considered introns to be one of the main factors contributing to the size variation of . The introns of the gene experienced frequent gain/loss events in ; and the intron position class cox1P386 was lost in the six ectomycorrhizal species. In addition, ectomycorrhizal species had more repetitive sequences and fewer intergenic sequences than asymbiotic species in their mitogenomes. Large-scale gene rearrangements were detected in the species we tested, including gene displacements and inversions. On the basis of the combined mitochondrial gene set, we reconstructed the phylogenetic relationships of 66 . The six ectomycorrhizal species were of single origin, and the two saprophytic species formed two distinct clades. This study is the first to elucidate the functions of the mitogenome in the evolution and ecological adaptation of species.
在本研究中，我们基于二代测序数据组装并分析了两种非共生和六种外生菌根真菌的线粒体基因组。八个线粒体基因组的大小在37,341至137,428 bp之间，我们认为内含子是导致其大小变异的主要因素之一。该基因的内含子在……中经历了频繁的获得/丢失事件；并且内含子位置类别cox1P386在六种外生菌根真菌中丢失。此外，外生菌根真菌的线粒体基因组中重复序列比非共生真菌更多，基因间序列更少。在我们测试的……物种中检测到大规模的基因重排，包括基因移位和倒位。基于合并的线粒体基因集，我们重建了66种……的系统发育关系。六种外生菌根真菌是单起源的，两种腐生真菌形成两个不同的分支。本研究首次阐明了线粒体基因组在……物种进化和生态适应中的功能。
