Liu Chao, Li Wan-Ying, Zheng Le-Xuan, Dao Mi, Chen Huan-Huan, Han Li-Hong
College of Biological Resource and Food Engineering, Yunnan Engineering Research Center of Fruit Wine, Qujing Normal University, Qujing, Yunnan, 655011, China Qujing Normal University Qujing China.
IMA Fungus. 2025 Feb 17;16:e141848. doi: 10.3897/imafungus.16.141848. eCollection 2025.
The genus , representing a diverse and widespread group of ectomycorrhizal mushroom-forming fungi, plays a crucial ecological and economical role. However, until now, a comprehensive description of its mitochondrial genome (mitogenome) has been lacking. In our current study, we have successfully assembled and analysed the mitogenomes of five species. These mitogenomes span a range from 35,618 base pairs (bp) to 42,088 bp, exhibiting a higher nucleotide abundance of AT compared to GC. All five mitogenomes harbour 14 conserved protein-coding genes (PCGs), two ribosomal RNAs (rRNAs) and 24 transfer RNAs (tRNAs). Notably, the overall ratio of Ka/Ks for all PCGs was found to be less than 1.0, indicating that these genes have undergone purifying selection during evolution. Intriguingly, the mitogenomic comparison revealed two instances of gene re-arrangement, which were directly linked to the geographical distribution of the species. The concatenated mitochondrial PCGs (mtPCGs) and nuclear ribosomal DNA (nrDNA) phylogenies displayed a robust congruent topology at the family level. Specifically, the species clustered together and formed sister relationship with other species in the mtPCGs tree. In contrast, the species grouped at the base of the nrDNA tree when concerning . This study represents the first report on the mitogenomes of the genus, providing valuable insights into fungal evolution within .
该属代表了一类多样且分布广泛的形成外生菌根的蘑菇真菌，具有至关重要的生态和经济作用。然而，直到现在，其线粒体基因组（线粒体基因组）仍缺乏全面的描述。在我们目前的研究中，我们成功组装并分析了五个物种的线粒体基因组。这些线粒体基因组的长度范围从35,618个碱基对（bp）到42,088 bp，与GC相比，AT的核苷酸丰度更高。所有五个线粒体基因组都包含14个保守的蛋白质编码基因（PCG）、两个核糖体RNA（rRNA）和24个转移RNA（tRNA）。值得注意的是，所有PCG的Ka/Ks总体比率小于1.0，这表明这些基因在进化过程中经历了纯化选择。有趣的是，线粒体基因组比较揭示了两例基因重排，这与该属物种的地理分布直接相关。串联的线粒体PCG（mtPCG）和核糖体DNA（nrDNA）系统发育在科级水平上显示出稳健的一致拓扑结构。具体而言，在mtPCG树中，该属物种聚集在一起，并与其他属物种形成姐妹关系。相比之下，在nrDNA树中，该属物种在涉及某个情况时聚集在基部。这项研究代表了关于该属线粒体基因组的首次报告，为该属内的真菌进化提供了有价值的见解。
