采用不对称眼底后照法的角膜和晶状体显微镜检查
corneal and lenticular microscopy with asymmetric fundus retroillumination.
作者信息
Weber Timothy D, Mertz Jerome
机构信息
Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.
Photonics Center, Boston University, 8 Saint Mary's Street, Boston, MA 02215, USA.
出版信息
Biomed Opt Express. 2020 May 26;11(6):3263-3273. doi: 10.1364/BOE.391815. eCollection 2020 Jun 1.
Abstract
We describe a new technique for non-contact corneal and lenticular microscopy. It is based on fundus retro-reflection and back-illumination of the crystalline lens and cornea. To enhance phase-gradient contrast, we apply asymmetric illumination by illuminating one side of the fundus. The technique produces micron-scale lateral resolution images across a 1 mm diagonal field of view in the central cornea. We show representative images of the epithelium, the subbasal nerve plexus, large stromal nerves, dendritic immune cells, endothelial nuclei, and the anterior crystalline lens, demonstrating the potential of this instrument for clinical applications.
摘要
我们描述了一种用于非接触式角膜和晶状体显微镜检查的新技术。它基于眼底的逆行反射以及晶状体和角膜的背向照明。为了增强相位梯度对比度，我们通过照亮眼底的一侧来应用非对称照明。该技术在中央角膜的1毫米对角视野范围内产生微米级横向分辨率图像。我们展示了上皮细胞、基底神经丛、大的基质神经、树突状免疫细胞、内皮细胞核和晶状体前部的代表性图像，证明了该仪器在临床应用中的潜力。
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