Anthony L S, James R F, Maudsley D J, Kongshavn P A
Department of Physiology, McGill University, Montreal, Quebec, Canada.
Immunology. 1988 Oct;65(2):273-6.
In this study, the effects of a monoclonal antibody, AF3.44.4, which is directed against antigen-specific, T-cell-derived helper factors, were investigated in two models of T-cell-macrophage co-operation. In vitro, cultures of nylon-wool-enriched peritoneal cells from mice immune to Listeria monocytogenes (T cells), macrophages from normal mice and Listeria antigen produced an activity mitogenic for mouse thymocytes (TMF). When AF3.44.4 was added to such cultures, the production of this activity was inhibited in a dose-dependent fashion. Preincubation of T cells with AF3.44.4, followed by its removal, was not sufficient for the inhibitory effect to be manifested. In an in vivo system, when spleen cells from Listeria-immunized mice were transferred to unprimed mice, the latter were protected against subsequent challenge with Listeria. Treatment of spleen cells with AF3.44.4 prior to transfer did not affect their ability to confer resistance upon recipients. It is postulated that AF3.44.4 acts on a population of T cells that is involved in macrophage-T-cell co-operation in vitro and that this population may be different from that involved in macrophage T-cell co-operation in vivo.
在本研究中，针对抗原特异性、T细胞衍生的辅助因子的单克隆抗体AF3.44.4的作用，在两种T细胞-巨噬细胞合作模型中进行了研究。在体外，来自对单核细胞增多性李斯特菌免疫的小鼠的经尼龙毛富集的腹膜细胞（T细胞）、正常小鼠的巨噬细胞和李斯特菌抗原的培养物产生了对小鼠胸腺细胞有丝分裂原活性的物质（TMF）。当将AF3.44.4添加到此类培养物中时，该活性的产生呈剂量依赖性受到抑制。用AF3.44.4对T细胞进行预孵育，然后去除该抗体，不足以使抑制作用显现出来。在体内系统中，当将来自经李斯特菌免疫的小鼠的脾细胞转移到未致敏的小鼠时，后者受到保护，免受随后的李斯特菌攻击。在转移前用AF3.44.4处理脾细胞并不影响它们赋予受体抗性的能力。据推测，AF3.44.4作用于体外参与巨噬细胞-T细胞合作的一群T细胞，并且这群细胞可能与体内参与巨噬细胞-T细胞合作的细胞不同。
