Näher H, Sperling U, Hahn H
J Immunol. 1985 Jan;134(1):569-72.
Cellular, genetic, and antigenic requirements for granuloma formation in murine listeriosis were determined by using adoptive transfer of granuloma formation. Granuloma formation was restricted by a class I MHC antigen (H-2K) and critically depended on a Ly-2+ (Ly-1+2+) T cell. Expression of granuloma formation required living bacteria; heat-killed bacteria was not sufficient. H-2K-restricted transfer of granuloma formation was associated with a high degree of protection. Markedly less protection, presumably due to macrophage activation by T cells, was found under conditions of H-2 I-A homology. It is concluded that two T cell populations are involved in protection against L. monocytogenes: protection associated with granuloma formation depends on Ly-2+ (Ly-1+2+) T cells, is restricted by H-2K, and requires products of living bacteria to be expressed, whereas protection based on macrophage activation depends on H-2 I-A-restricted T helper cells.
通过采用肉芽肿形成的过继转移方法，确定了小鼠李斯特菌病中肉芽肿形成的细胞、遗传和抗原需求。肉芽肿的形成受I类主要组织相容性复合体（MHC）抗原（H-2K）限制，并且严重依赖Ly-2+（Ly-1+2+）T细胞。肉芽肿形成的表达需要活细菌；热灭活细菌则不足以引发。H-2K限制的肉芽肿形成转移与高度保护相关。在H-2 I-A同源的条件下，发现保护作用明显较弱，推测这是由于T细胞激活巨噬细胞所致。结论是，有两种T细胞群体参与抵抗单核细胞增生李斯特菌的保护作用：与肉芽肿形成相关的保护作用依赖于Ly-2+（Ly-1+2+）T细胞，受H-2K限制，并且需要活细菌的产物得以表达，而基于巨噬细胞激活的保护作用则依赖于H-2 I-A限制的T辅助细胞。
