Kallenberg C G, van Dissel-Emiliani F, Huitema M G, Limburg P C, The T H
Department of Clinical Immunology, University Hospital, Groningen, The Netherlands.
J Clin Lab Immunol. 1988 Jun;26(2):55-61.
Systemic lupus erythematosus (SLE) and mixed connective tissue disease (MCTD) are characterized by B-cell hyperactivity as manifested by spontaneous in vitro production of immunoglobulins (Ig), but pokeweed mitogen (PWM)-induced Ig synthesis of peripheral blood (PB) mononuclear cells, a T-cell dependent process, is markedly decreased. We analyzed the defective capacity to produce Ig in vitro in 11 patients with SLE and 11 with MCTD. PWM-activated PB T-cells, both from SLE- and MCTD-patients, did not differ from control T-cells in their capacity to produce B-cell growth factors (BCGF) and B-cell differentiation factors (BCDF). However, B-cell proliferative responsiveness to control T-cell factors was decreased compared to control B-cells (p less than 0.01). In contrast to PB B-cells splenic B-cells from a patient with MCTD who underwent splenectomy were highly responsive to T-cell factors in a dose dependent way. It is concluded that the defective PWM-induced Ig production of PB lymphocytes in SLE/MCTD is due to decreased responsiveness of PB B cells to T-cell factors. However, analysis of splenic lymphocytes may be more representative for evaluation of the immunoregulatory disturbances in these disorders.
系统性红斑狼疮（SLE）和混合性结缔组织病（MCTD）的特征是B细胞功能亢进，表现为体外自发产生免疫球蛋白（Ig），但T细胞依赖性过程——外周血（PB）单个核细胞的商陆丝裂原（PWM）诱导的Ig合成明显减少。我们分析了11例SLE患者和11例MCTD患者体外产生Ig的缺陷能力。来自SLE和MCTD患者的PWM激活的PB T细胞在产生B细胞生长因子（BCGF）和B细胞分化因子（BCDF）的能力方面与对照T细胞没有差异。然而，与对照B细胞相比，B细胞对对照T细胞因子的增殖反应性降低（p<0.01）。与PB B细胞相反，一名接受脾切除术的MCTD患者的脾B细胞以剂量依赖方式对T细胞因子高度反应。结论是，SLE/MCTD中PB淋巴细胞PWM诱导的Ig产生缺陷是由于PB B细胞对T细胞因子的反应性降低。然而，脾淋巴细胞分析可能更能代表评估这些疾病中的免疫调节紊乱。
