Pham B N, Prin L, Gosset D, Hatron P Y, Devulder B, Capron A, Dessaint J P
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167-CNRS 624, Institut Pasteur de Lille, France.
Clin Exp Immunol. 1989 Aug;77(2):168-74.
Polyclonal B cell activity in systemic lupus erythematosus (SLE) may be under T cell control. The use of nitrocellulose immunoblots for the analysis of recognition by peripheral blood lymphocytes of nucleoplasmic proteins in SLE patients led to the characterization of significant proliferative responses to 68K (U1 RNP); SS-B; B-B' and D (Sm) antigen in 15 of 20 patients. Variations of proliferative response were parallel to disease activity over a follow-up period of greater than or equal to 6 months, conferring some prognostic value to the assay of lymphocyte response to nucleoplasmic antigens. The pattern of reactivity differs from the corresponding serum antibody profile, and purified T cell suspensions (greater than 95% pure) were shown to proliferate in response to soluble nucleoplasmic antigens, indicating that T and B cell repertoires against nucleoplasmic proteins may differ. This suggests that activated helper T cells contribute to the fine modulation of B cell reactivity to subcellular particles to determine the particular antibody profile of the patients.
系统性红斑狼疮（SLE）中的多克隆B细胞活性可能受T细胞控制。使用硝酸纤维素免疫印迹法分析SLE患者外周血淋巴细胞对核质蛋白的识别，结果显示20例患者中有15例对68K（U1 RNP）、SS - B、B - B'和D（Sm）抗原产生显著的增殖反应。在大于或等于6个月的随访期内，增殖反应的变化与疾病活动平行，这赋予了淋巴细胞对核质抗原反应性检测一定的预后价值。反应模式不同于相应的血清抗体谱，并且纯化的T细胞悬液（纯度大于95%）显示可对可溶性核质抗原产生增殖反应，表明针对核质蛋白的T细胞和B细胞库可能不同。这表明活化的辅助性T细胞有助于精细调节B细胞对亚细胞颗粒的反应性，从而确定患者的特定抗体谱。
