Department of Grassland Science, Sichuan Agricultural University, Chengdu, 6111130, China.
Herbivorous Livestock Research Institute, Chongqing Academy of Animal Sciences, Chongqing, China.
BMC Plant Biol. 2020 Jul 8;20(1):323. doi: 10.1186/s12870-020-02530-0.
Heat and drought are serious threats for crop growth and development. As the sixth largest cereal crop in the world, pearl millet can not only be used for food and forage but also as a source of bioenergy. Pearl millet is highly tolerant to heat and drought. Given this, it is considered an ideal crop to study plant stress tolerance and can be used to identify heat-resistant genes.
In this study, we used Pacbio sequencing data as a reference sequence to analyze the Illumina data of pearl millet that had been subjected to heat and drought stress for 48 h. By summarizing previous studies, we found 26,299 new genes and 63,090 new transcripts, and the number of gene annotations increased by 20.18%. We identified 2792 transcription factors and 1223 transcriptional regulators. There were 318 TFs and 149 TRs differentially expressed under heat stress, and 315 TFs and 128 TRs were differentially expressed under drought stress. We used RNA sequencing to identify 6920 genes and 6484 genes differentially expressed under heat stress and drought stress, respectively.
Through Pacbio sequencing, we have identified more new genes and new transcripts. On the other hand, comparing the differentially expressed genes under heat tolerance with the DEGs under drought stress, we found that even in the same pathway, pearl millet responds with a different protein.
热胁迫和干旱是影响作物生长和发育的严重威胁。珍珠粟是世界第六大谷类作物，不仅可作为食物和饲料，还可用作生物能源的来源。珍珠粟高度耐受热胁迫和干旱胁迫。鉴于此，它被认为是研究植物抗逆性的理想作物，可以用来鉴定耐热基因。
本研究以 Pacbio 测序数据为参考序列，分析了珍珠粟在经历 48 小时热胁迫和干旱胁迫后的 Illumina 数据。通过总结前人的研究，我们发现了 26299 个新基因和 63090 个新转录本，基因注释数量增加了 20.18%。我们鉴定了 2792 个转录因子和 1223 个转录调控因子。在热胁迫下有 318 个 TF 和 149 个 TR 差异表达，在干旱胁迫下有 315 个 TF 和 128 个 TR 差异表达。我们使用 RNA 测序分别鉴定了 6920 个和 6484 个在热胁迫和干旱胁迫下差异表达的基因。
通过 Pacbio 测序，我们鉴定了更多的新基因和新转录本。另一方面，将耐热条件下差异表达的基因与干旱胁迫下差异表达的基因进行比较，我们发现即使在同一通路中，珍珠粟也会产生不同的蛋白来响应胁迫。
