Burklund Alison, Petryk James D, Hoopes P Jack, Zhang John X J
Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA.
Geisel School of Medicine, Dartmouth College, Hanover, New Hampshire 03755, USA.
Biomicrofluidics. 2020 Jun 23;14(3):034115. doi: 10.1063/5.0011908. eCollection 2020 May.
We report on a microsystem that couples high-throughput bacterial immunomagnetic capture to contact-free cell lysis using an alternating current magnetic field (AMF) to enable downstream molecular characterization of bacterial nucleic acids. Traditional methods for cell lysis rely on either dilutive chemical methods, expensive biological reagents, or imprecise physical methods. We present a microchip with a magnetic polymer substrate (Mag-Polymer microchip), which enables highly controlled, on-chip heating of biological targets following exposure to an AMF. First, we present a theoretical framework for the quantitation of power generation for single-domain magnetic nanoparticles embedded in a polymer matrix. Next, we demonstrate successful bacterial DNA recovery by coupling (1) high-throughput, sensitive microfluidic immunomagnetic capture of bacteria to (2) on-chip, contact-free bacterial lysis using an AMF. The bacterial capture efficiency exceeded 76% at 50 ml/h at cell loads as low as ∼10 CFU/ml, and intact DNA was successfully recovered at starting bacterial concentrations as low as ∼1000 CFU/ml. Using the presented methodology, cell lysis becomes non-dilutive, temperature is precisely controlled, and potential contamination risks are eliminated. This workflow and substrate modification could be easily integrated in a range of micro-scale diagnostic systems for infectious disease.
我们报道了一种微系统,该系统将高通量细菌免疫磁捕获与使用交变电流磁场(AMF)的非接触式细胞裂解相结合,以实现细菌核酸的下游分子表征。传统的细胞裂解方法要么依赖于稀释化学方法、昂贵的生物试剂,要么依赖于不精确的物理方法。我们展示了一种具有磁性聚合物基质的微芯片(磁聚合物微芯片),在暴露于AMF后,它能够对生物靶点进行高度可控的芯片上加热。首先,我们提出了一个理论框架,用于定量嵌入聚合物基质中的单畴磁性纳米颗粒的发电情况。接下来,我们通过将(1)高通量、灵敏的微流控细菌免疫磁捕获与(2)使用AMF的芯片上非接触式细菌裂解相结合,证明了成功回收细菌DNA。在细胞负载低至约10 CFU/ml的情况下,流速为50 ml/h时细菌捕获效率超过76%,在起始细菌浓度低至约1000 CFU/ml时成功回收了完整的DNA。使用所提出的方法,细胞裂解变得无需稀释,温度得到精确控制,并且消除了潜在的污染风险。这种工作流程和底物修饰可以很容易地集成到一系列用于传染病的微型诊断系统中。