Al-Abbasi Fahad A
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Saudi J Biol Sci. 2021 Jan;28(1):566-571. doi: 10.1016/j.sjbs.2020.10.042. Epub 2020 Oct 29.
Regular exposure to stress causes alteration in biochemical parameter but till date no specific medicine prescribed for controlling it. Current study aimed to determine the effect of Diazepam on proinflammatory and cardiac markers in stress exposed rats. Male Wistar rats were divided into four groups with six animals in each group for 90 days study. Group-1 served as a Normal Control (NC), Groups-2, as a Disease Control (DC), Group-3 as a Diazepam Control (DMC) and Group-4 as a Disease + Diazepam Treatment (DT). DMC and DT animals exposed to regular stress by forced swimming exercise method for 90 days. DMC and DT received 5 mg/kg, p.o the daily dose of Diazepam. At the end of the protocol, animals were sacrificed. The level of serum proinflammatory marker interleukin-6 in DC increased significantly (p < 0.001) while restored significantly (p < 0.001) in DT. Level of interleukin-10 in DC decreased significantly (p < 0.001) while restored significantly (p < 0.001) in DT. Level of fibrinogen was also increased by stress, which was restored significantly (p < 0.05) by diazepam. Increased level of Creatine kinase-MB (CK-MB) by stress was restored significantly (p < 0.05) by diazepam. The level of cortisol was increased also significantly (p < 0.001) and restored to normal by diazepam. The level of C-reactive protein (CRP) and cholesterol was increased significantly (p < 0.01; p < 0.001) by stress while restored significantly (p < 0.01; p < 0.001) by diazepam. Findings from results suggest that diazepam ameliorates altered proinflammatory and cardiac markers in stress exposed rats.
经常暴露于应激状态会导致生化参数改变，但迄今为止尚无专门用于控制应激的药物。当前研究旨在确定地西泮对应激暴露大鼠促炎和心脏标志物的影响。将雄性Wistar大鼠分为四组，每组六只动物，进行为期90天的研究。第1组作为正常对照组（NC），第2组作为疾病对照组（DC），第3组作为地西泮对照组（DMC），第4组作为疾病+地西泮治疗组（DT）。通过强迫游泳运动方法使DMC和DT组动物暴露于常规应激状态90天。DMC和DT组动物每日口服5mg/kg地西泮。实验方案结束时，处死动物。DC组血清促炎标志物白细胞介素-6水平显著升高（p<0.001），而DT组显著恢复（p<0.001）。DC组白细胞介素-10水平显著降低（p<0.001），而DT组显著恢复（p<0.001）。应激还会使纤维蛋白原水平升高，地西泮使其显著恢复（p<0.05）。应激导致的肌酸激酶同工酶（CK-MB）水平升高被地西泮显著恢复（p<0.05）。皮质醇水平也显著升高（p<0.001），地西泮使其恢复正常。应激使C反应蛋白（CRP）和胆固醇水平显著升高（p<0.01；p<0.001），而地西泮使其显著恢复（p<0.01；p<0.001）。结果表明，地西泮可改善应激暴露大鼠促炎和心脏标志物的改变。
