UCIBIO/REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
ESALD, Instituto Politécnico de Castelo Branco, Castelo Branco, Portugal.
Eur J Clin Microbiol Infect Dis. 2020 Dec;39(12):2471-2475. doi: 10.1007/s10096-020-03956-y. Epub 2020 Jul 8.
The spread of multidrug-resistant (MDR) Klebsiella pneumoniae in the nosocomial setting represents a big challenge to infection control teams. We have recently developed a simple spectroscopic-based method with excellent accuracy, turnaround time and cost-effectiveness (Rodrigues et al. mSystems 2020) for bacterial typing. Here, we applied our method in a real clinical context to support early identification of an outbreak involving KPC-3-producing K. pneumoniae ST147 isolates. Our results further support that attenuated total reflectance Fourier transform infrared (FT-IR) spectroscopy can provide enough information to support early and adequate infection control measures and therapeutic choices in the context of nosocomial outbreaks and hospital surveillance.
在医院环境中，多重耐药（MDR）肺炎克雷伯菌的传播对感染控制团队来说是一个巨大的挑战。我们最近开发了一种基于光谱的简单方法，具有出色的准确性、周转时间和成本效益（Rodrigues 等人，mSystems 2020），用于细菌分型。在这里，我们将我们的方法应用于真实的临床环境中，以支持对涉及产 KPC-3 的肺炎克雷伯菌 ST147 分离株的暴发的早期识别。我们的结果进一步支持衰减全反射傅里叶变换红外（FT-IR）光谱可以提供足够的信息，以支持在医院暴发和医院监测的情况下进行早期和充分的感染控制措施和治疗选择。
