School of Life Sciences, Tiangong University, Xiqing District, Binshui West Road, Tianjin, 300387, China.
Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China.
Lasers Med Sci. 2021 Apr;36(3):555-562. doi: 10.1007/s10103-020-03057-4. Epub 2020 Jul 9.
Delayed wound healing is one of the most challenging complications of diabetes mellitus (DM) in clinical medicine, and it is related to the excessive generation of reactive oxygen species (ROS). Photobiomodulation (PBM) can promote wound healing in many ways, so it can be used as a method for the treatment of delayed healing of DM wounds. In this study, we investigated the effect of PBM on ROS homeostasis in human embryonic skin fibroblast cells (CCC-ESFs) cultured in high glucose concentrations. The CCC-ESFs were cultured in vitro and divided into two groups, including the control group and the 635 nm laser irradiation group. After 2 days of high glucose treatment, the experimental group was irradiated with different doses of laser for 3 days. First, we measured the cellular proliferation, and the results showed that laser irradiation could promote cellular proliferation. Then, we measured the generation of ROS, the activities of total superoxide dismutase (SOD), and total antioxidant capacity (TAC) of the cells; the results showed that high glucose destroyed cells by inducing high concentration of ROS, the balance of oxidation, and antioxidation cause oxidative stress damage to cells. PBM can increase the antioxidant capacity of cells, reducing the high concentration of ROS induced by high glucose. Finally, we measured the levels of mitochondrial membrane potential (∆ψ) and the secretion of nuclear factor kappa-B (NF-κB), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β); the results showed that PBM can reduce apoptosis and regulate the inflammatory state. We conclude that PBM can maintain the ROS homeostasis, increase the TAC of cells, and trigger the cellular proliferation, and the response of CCC-ESFs to PBM was dose-dependent.
伤口愈合延迟是临床医学中糖尿病（DM）最具挑战性的并发症之一，这与活性氧（ROS）的过度产生有关。光生物调节（PBM）可以通过多种方式促进伤口愈合，因此可以用作治疗 DM 伤口愈合延迟的方法。在这项研究中，我们研究了 PBM 对高糖浓度培养的人胚胎皮肤成纤维细胞（CCC-ESFs）中 ROS 动态平衡的影响。CCC-ESFs 在体外培养，并分为对照组和 635nm 激光照射组。高糖处理 2 天后，实验组用不同剂量的激光照射 3 天。首先，我们测量了细胞增殖，结果表明激光照射可以促进细胞增殖。然后，我们测量了细胞中 ROS 的产生、总超氧化物歧化酶（SOD）的活性和总抗氧化能力（TAC）；结果表明，高糖通过诱导高浓度的 ROS、氧化和抗氧化的平衡破坏细胞，导致细胞氧化应激损伤。PBM 可以增加细胞的抗氧化能力，减少高糖诱导的高浓度 ROS。最后，我们测量了线粒体膜电位（∆ψ）和核因子 kappa-B（NF-κB）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的分泌水平；结果表明，PBM 可以减少细胞凋亡并调节炎症状态。我们得出结论，PBM 可以维持 ROS 动态平衡，增加细胞 TAC，并触发 CCC-ESFs 的细胞增殖，而 CCC-ESFs 对 PBM 的反应是剂量依赖性的。
