Department of Periodontology, Faculty of Dentistry, University of Marmara, 34854 Maltepe, Istanbul, Turkey.
Department of Periodontology, Institute of Dentistry, University of Turku, Turku, Finland.
Clin Oral Investig. 2021 Mar;25(3):1117-1126. doi: 10.1007/s00784-020-03411-4. Epub 2020 Jul 8.
Angiogenesis is essential in maintenance of periodontal homeostasis, and it is regulated by growth factors and cytokines, including basic fibroblast growth factor (b-FGF), endoglin, platelet and endothelial cell adhesion molecule (PECAM-1), vascular endothelial growth factor (VEGF), soluble intercellular adhesion molecule-1 (sICAM-1), and soluble vascular cell adhesion molecule-1 (sVCAM-1). In this study, the salivary and serum concentrations of these angiogenesis-related proteins in relation to smoking and periodontitis were examined.
Full-mouth periodontal status together with unstimulated whole saliva and serum samples was collected from 78 individuals, including 40 periodontitis patients (20 smokers and 20 nonsmokers) and 38 periodontally healthy controls (20 smokers and 18 nonsmokers). The Luminex®-xMAP™ technique was used for protein analyses.
Concentrations of all tested proteins in saliva as well as VEGF in serum were significantly higher in periodontitis patients than in healthy controls. In smokers, serum concentrations of endoglin (p = 0.017) and sICAM-1 (p = 0.001) were elevated in comparison to nonsmokers. After adjusting for smoking and gender, periodontitis associated significantly with salivary concentrations of b-FGF, PECAM-1, VEGF, sICAM-1, and sVCAM-1 (p < 0.01).
Taken together, salivary concentrations of b-FGF, PECAM-1, and VEGF associate with periodontitis. The suppressive effect of smoking on salivary marker levels is limited to periodontitis patients only.
Smoking-related suppression of salivary marker levels is observed only in periodontitis patients.
血管生成对于维持牙周组织稳态至关重要，它受到生长因子和细胞因子的调节，包括碱性成纤维细胞生长因子（b-FGF）、内皮糖蛋白、血小板内皮细胞黏附分子（PECAM-1）、血管内皮生长因子（VEGF）、可溶性细胞间黏附分子-1（sICAM-1）和可溶性血管细胞黏附分子-1（sVCAM-1）。本研究旨在探讨这些与血管生成相关的蛋白在唾液和血清中的浓度与吸烟和牙周炎的关系。
从 78 名个体中采集全口牙周状况以及未刺激的全唾液和血清样本，其中包括 40 名牙周炎患者（20 名吸烟者和 20 名非吸烟者）和 38 名牙周健康对照者（20 名吸烟者和 18 名非吸烟者）。采用 Luminex®-xMAP™技术进行蛋白分析。
与健康对照组相比，牙周炎患者的唾液中所有检测蛋白的浓度以及血清中 VEGF 的浓度均显著升高。与非吸烟者相比，吸烟者血清中内皮糖蛋白（p=0.017）和 sICAM-1（p=0.001）的浓度升高。在调整了吸烟和性别因素后，牙周炎与唾液中 b-FGF、PECAM-1、VEGF、sICAM-1 和 sVCAM-1 的浓度显著相关（p<0.01）。
唾液中 b-FGF、PECAM-1 和 VEGF 的浓度与牙周炎有关。吸烟对唾液标志物水平的抑制作用仅局限于牙周炎患者。
仅在牙周炎患者中观察到与吸烟相关的唾液标志物水平抑制。
