Rothman P, Lutzker S, Cook W, Coffman R, Alt F W
Howard Hughes Medical Institute, New York, New York.
J Exp Med. 1988 Dec 1;168(6):2385-9. doi: 10.1084/jem.168.6.2385.
To elucidate the mechanism of IL-4-induced enhancement of IgE and IgG1 production, murine splenic B cells and A-MuLV-transformed cells were cultured with LPS and IL-4 and assayed for epsilon and gamma 1 transcripts. Concomitant treatment with IL-4 and LPS induced expression of C epsilon transcripts in both normal and transformed cells. Expression of these truncated C epsilon transcripts preceded accumulation of normal epsilon mRNA in treated cells. Consistent data were obtained with respect to gamma 1 RNA expression. These results suggest that IL-4 can direct class switching in the context of a mechanism associated with differential expression of germline constant region genes.
为阐明白细胞介素-4（IL-4）诱导IgE和IgG1产生增强的机制，将小鼠脾脏B细胞和A-MuLV转化细胞与脂多糖（LPS）和IL-4一起培养，并检测ε和γ1转录本。IL-4和LPS联合处理诱导正常细胞和转化细胞中Cε转录本的表达。这些截短的Cε转录本的表达先于处理细胞中正常εmRNA的积累。关于γ1RNA表达也获得了一致的数据。这些结果表明，IL-4可在与种系恒定区基因差异表达相关的机制背景下指导类别转换。
