Radbruch A, Sablitzky F
EMBO J. 1983;2(11):1929-35. doi: 10.1002/j.1460-2075.1983.tb01681.x.
Mouse B lymphocytes can be activated polyclonally by bacterial lipopolysaccharide (LPS) to differentiate into plasmablasts. Within several days many cells perform immunoglobulin (Ig) class switching in vitro. We have purified LPS blasts expressing IgM or only IgG3 on the cell surface and analysed the DNA of these cells by Southern hybridisation blotting to detect rearrangement or deletion of CH genes. Quantitative evaluation of the Southern blots suggests that populations of surface IgG3+ (sIgG3+) cells from 6-day and sIgM+ cells from 8-day-old cultures contain only about half as many Cmu genes as spleen cells. Cmu deletion is nearly complete in populations of sIgG3+ cells from 9-day-old cultures. Therefore, upon stimulation with LPS, within a few days Cmu is deleted in most sIgG3+ cells from both chromosomes.
小鼠B淋巴细胞可被细菌脂多糖（LPS）多克隆激活，分化为成浆细胞。在几天内，许多细胞在体外进行免疫球蛋白（Ig）类别转换。我们纯化了在细胞表面表达IgM或仅表达IgG3的LPS刺激的母细胞，并通过Southern杂交印迹分析这些细胞的DNA，以检测CH基因的重排或缺失。对Southern印迹的定量评估表明，来自6日龄培养物的表面IgG3 +（sIgG3 +）细胞群体和来自8日龄培养物的sIgM +细胞群体中所含的Cmu基因数量仅约为脾细胞的一半。在来自9日龄培养物的sIgG3 +细胞群体中，Cmu缺失几乎完全。因此，在用LPS刺激后，在几天内，大多数来自两条染色体的sIgG3 +细胞中的Cmu被删除。
