Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, People's Republic of China.
Suzhou Maximum Bio-tech Co., Ltd., Suzhou, People's Republic of China.
Emerg Microbes Infect. 2020 Dec;9(1):1682-1691. doi: 10.1080/22221751.2020.1793689.
Ideal methods for detecting pathogens should be sensitive, specific, rapid, cost-effective and instrument-free. Conventional nucleic acid pathogen detection strategies, mostly PCR-based techniques, have various limitations, such as expensive equipment, reagents and skilled performance. Recently, CRISPR/Cas-based methods have burst onto the scene, with the potential to power the pathogen detection field. Here we introduce these unique methods and discuss its hurdles and promises.
理想的病原体检测方法应该具有灵敏度高、特异性强、快速、经济实惠且无需仪器的特点。传统的核酸病原体检测策略，主要是基于 PCR 的技术，存在各种局限性，如昂贵的设备、试剂和熟练的操作。最近，基于 CRISPR/Cas 的方法异军突起，有可能为病原体检测领域提供动力。在这里，我们介绍这些独特的方法，并讨论它们的障碍和前景。
