Biological Science Research Center, Southwest University, Chongqing 400716, China; Wound Trauma Medical Center, State Key Laboratory of Trauma, Burns, and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042, China.
Biological Science Research Center, Southwest University, Chongqing 400716, China; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
Trends Biotechnol. 2022 Nov;40(11):1326-1345. doi: 10.1016/j.tibtech.2022.04.006. Epub 2022 May 17.
An ideal molecular diagnostic method should be sensitive, specific, low cost, rapid, portable, and easy to operate. Traditional nucleic acid detection methods based mainly on PCR technology have not only high sensitivity and specificity, but also some limitations, such as the need for expensive equipment and skilled technicians, being both time and labor intensive, and difficult to implement in some regions. However, with the continuous development of CRISPR-Cas technology and its application in molecular diagnosis, new approaches have been used for the construction of molecular diagnostic systems. In this review, we discuss recent advances in CRISPR-based molecular diagnostic technologies and highlight the revolution they bring to the field of molecular diagnostics.
理想的分子诊断方法应具有灵敏度高、特异性强、成本低、快速、便携和易于操作等特点。传统的基于 PCR 技术的核酸检测方法不仅具有高灵敏度和特异性，而且还存在一些局限性，例如需要昂贵的设备和熟练的技术人员，既费时又费力，并且在某些地区难以实施。然而，随着 CRISPR-Cas 技术的不断发展及其在分子诊断中的应用，已经开发出了一些新的方法来构建分子诊断系统。在这篇综述中，我们讨论了基于 CRISPR 的分子诊断技术的最新进展，并强调了它们给分子诊断领域带来的变革。
