Centre of Biotechnology, University of Allahabad, Prayagraj 211002, India.
Department of Physics, University of Allahabad, Prayagraj 211002, India.
Molecules. 2020 Jul 7;25(13):3091. doi: 10.3390/molecules25133091.
Herbal medicines are widely used worldwide and much appreciated because of their fewer side effects and the ability to fight diseases at the root cause. Active 'phyto' ingredients require a scientific approach and a mechanism to distribute components at the target site for better therapeutic results. Nanotechnology, on the other hand, has created new hope for cancer treatment but is still far from being proven in clinical settings. This article combines a unique approach to synthesis with the use of , followed by microwave irritation of silver and gold nanoparticles that ensures the capping of the active phyto ingredient and further enhances the effects of nanomedicine to fight colon cancer, thus opening a new era of what we call herbonanoceutics. The article also compares the characteristics and properties of silver (Au) and gold (Ag) nanoparticles synthesized by an in house developed novel microwave-assisted rapid green synthesis method. The as-prepared Ag NPs and Au NPs were compared using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and energy dispersive spectroscopy (EDS). Our comparative study revealed that both assemblies display face-centred cubic structures (FCCs) and are nanocrystalline in nature. The advantage of the approach was that the sizes of gold and silver were identical in range with a similar distribution pattern. This has helped us to study the activity against colon cancer cell line (HCT-116) without incoherence since size plays a key role in the application. More specifically, morphological changes, cell viability, the production of reactive oxygen species (ROS) and the fragmentation of DNA have been further reported to assess better the results obtained with the two metals. Our results suggest that the newly adopted synthesis method may ensure the dual benefits from phyto ingredients which further enhances the effectiveness of advanced nanomedicine.
草药在世界范围内被广泛应用，因其副作用少且能够从根本上治疗疾病而备受推崇。活性“植物”成分需要一种科学的方法和一种将成分分布到靶位的机制，以获得更好的治疗效果。另一方面，纳米技术为癌症治疗带来了新的希望，但在临床环境中仍远未得到证实。本文将独特的合成方法与 相结合，随后对银和金纳米粒子进行微波刺激，确保了活性植物成分的封闭，并进一步增强了纳米医学对抗结肠癌的效果，从而开创了我们称之为“herbonanoceutics”的新时代。本文还比较了通过自行开发的新型微波辅助快速绿色合成方法合成的银（Au）和金（Ag）纳米粒子的特性和性质。使用紫外-可见光谱（UV-Vis）、傅里叶变换红外（FTIR）光谱、X 射线衍射（XRD）、透射电子显微镜（TEM）和能量色散光谱（EDS）对 Ag NPs 和 Au NPs 进行了比较。我们的比较研究表明，两种组装体都显示出面心立方结构（FCCs），且本质上是纳米晶。该方法的优点是金和银的尺寸范围相同，分布模式相似。这有助于我们在没有不和谐的情况下研究对结肠癌细胞系（HCT-116）的活性，因为尺寸在应用中起着关键作用。更具体地说，形态变化、细胞活力、活性氧（ROS）的产生和 DNA 的片段化已进一步报道，以更好地评估两种金属获得的结果。我们的结果表明，新采用的合成方法可以确保从植物成分中获得双重益处，从而进一步增强先进纳米医学的效果。
