Division of Children's and Women Health (BKH), Obstetrics and Gynecology, Department of Biomedical and Clinical Sciences (BKV), Linköping University, 58185 Linköping, Sweden.
Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Genes (Basel). 2020 Jul 7;11(7):758. doi: 10.3390/genes11070758.
Semen changes the gene expression in endometrial and oviductal tissues modulating important processes for reproduction. We tested the hypothesis that mating and/or sperm-free seminal plasma deposition in the reproductive tract affect the expression of genes associated with sperm-lining epithelium interactions, ovulation, and pre-implantation effects (nerve growth factor, NGF; α/β hydrolase domain-containing protein 2, ABHD2; C-terminal tensin-like protein, CTEN or TNS4; and versican, VCAN) in the period 10-72 h post-mating. In Experiment 1, does ( = 9) were treated with gonadotropin-releasing hormone (GnRH) (control), GnRH-stimulated, and vaginally infused with sperm-free seminal plasma (SP-AI), or GnRH-stimulated and naturally mated (NM). In Experiment 2, does ( = 15) were GnRH-stimulated and naturally mated. Samples were retrieved from the internal reproductive tracts (cervix-to-infundibulum) 20 h post-treatment (Experiment 1) or sequentially collected at 10, 24, 36, 68, or 72 h post-mating (Experiment 2, 3 does/period). All samples were processed for gene expression analysis by quantitative PCR. Data showed an upregulation of endometrial and by NM, but not by SP-AI. The findings suggest that the gene affects the reproductive tract of the doe during ovulation and beyond, influencing the maternal environment during early embryonic development.
精液改变了子宫内膜和输卵管组织的基因表达，调节了生殖的重要过程。我们检验了这样一个假设，即交配和/或精子自由的精液在生殖道中的沉积会影响与精子衬里上皮相互作用、排卵和植入前效应（神经生长因子，NGF；α/β水解酶结构域蛋白 2，ABHD2；C 端张力蛋白样蛋白，CTEN 或 TNS4；和 versican，VCAN）相关的基因的表达，这些基因的表达发生在交配后 10-72 小时。在实验 1 中，9 只母羊接受了促性腺激素释放激素（GnRH）处理（对照组）、GnRH 刺激和阴道内注入精子自由的精液（SP-AI）或 GnRH 刺激和自然交配（NM）。在实验 2 中，15 只母羊接受了 GnRH 刺激和自然交配。在治疗后 20 小时（实验 1）或在交配后 10、24、36、68 或 72 小时（实验 2，3 只/期），从内部生殖道（宫颈到壶腹）中取出所有样本进行基因表达分析。所有样本均通过定量 PCR 进行基因表达分析。结果显示，NM 上调了子宫内膜和的表达，但 SP-AI 没有。这些发现表明，基因影响母羊在排卵和排卵后的生殖道，影响早期胚胎发育期间的母体环境。
