Zheng Youjing, He Jia-Qiang
Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061, USA.
EXCLI J. 2021 Jan 18;20:126-141. doi: 10.17179/excli2020-3262. eCollection 2021.
Coronary artery disease (CAD) and atrial fibrillation (AF) share common risk factors, such as hypertension and diabetes. The patients with CAD often suffer concomitantly AF, but how two diseases interact with each other at cellular and molecular levels remain largely unknown. The present study aims to dissect the common differentially expressed genes (DEGs) that are concurrently associated with CAD and AF. Two datasets [GSE71226 for CAD) and GSE31821 for AF] were analyzed with GEO2R and Venn Diagram to identify the DEGs. Signaling pathways, gene enrichments, and protein-protein interactions (PPI) of the identified common DEGs were further analyzed with Kyoto Encyclopedia of Gene and Genome (KEGG), Database for Annotation, Visualization and Integrated Discovery (DAVID), and Search Toll for the Retrieval of Interacting Genes (STRING). 565 up- and 1367 down-regulated genes in GSE71226 and 293 up- and 68 down-regulated genes in GSE31821 were identified. Among those, 21 common DEGs were discovered from both datasets, which lead to the findings of 4 CAD and 21 AF pathways, 3 significant gene enrichments (intracellular cytoplasm, protein binding, and vascular labyrinthine layer), and 3 key proteins (membrane metallo-endopeptidase (MME), transferrin receptor 1 (TfR1), and Lysosome-associated membrane glycoprotein 1 (LAMP1)). Together, these data implied that these three proteins may play a central role in development of both CAD and AF.
冠状动脉疾病（CAD）和心房颤动（AF）具有共同的危险因素，如高血压和糖尿病。CAD患者常并发AF，但这两种疾病在细胞和分子水平上如何相互作用仍 largely未知。本研究旨在剖析与CAD和AF同时相关的共同差异表达基因（DEG）。使用GEO2R和韦恩图分析了两个数据集[用于CAD的GSE71226和用于AF的GSE31821]以识别DEG。使用京都基因与基因组百科全书（KEGG）、注释、可视化和综合发现数据库（DAVID）以及用于检索相互作用基因的搜索工具（STRING）进一步分析了所识别的共同DEG的信号通路、基因富集和蛋白质-蛋白质相互作用（PPI）。在GSE71226中鉴定出565个上调基因和1367个下调基因，在GSE31821中鉴定出293个上调基因和68个下调基因。其中，从两个数据集中发现了21个共同的DEG，这导致发现了4条CAD和21条AF通路、3个显著的基因富集（细胞内细胞质、蛋白质结合和血管迷路层）以及3个关键蛋白（膜金属内肽酶（MME）、转铁蛋白受体1（TfR1）和溶酶体相关膜糖蛋白1（LAMP1））。总之，这些数据表明这三种蛋白质可能在CAD和AF的发展中起核心作用。
