Li Ying, Jiang Jiliang, Tong Liangcheng, Gao Tingting, Bai Lei, Xue Qing, Xing Jianxin, Wang Qin, Lyu Haoran, Cai Min, Sun Zhongyang
Department of Orthopedics, Air Force Hospital of Eastern Theater, Anhui Medical University, Nanjing, China.
Department of Neurosurgery, Yulin First Hospital, the Second Affiliated Hospital of Yan'an University, Yulin, China.
BMC Musculoskelet Disord. 2020 Jul 9;21(1):449. doi: 10.1186/s12891-020-03479-9.
Clinically, skeletal muscle ischemia/reperfusion injury is a life-threatening syndrome that is often caused by skeletal muscle damage and is characterized by oxidative stress and inflammatory responses. Bilobalide has been found to have antioxidative and anti-inflammatory effects. However, it is unclear whether bilobalide can protect skeletal muscle from ischemia/reperfusion injury.
The effects of bilobalide on ischemia/reperfusion-injured skeletal muscle were investigated by performing hematoxylin and eosin staining and assessing the wet weight/dry weight ratio of muscle tissue. Then, we measured lipid peroxidation, antioxidant activity and inflammatory cytokine levels. Moreover, Western blotting was conducted to examine the protein levels of MAPK/NF-휅B pathway members.
Bilobalide treatment could protected hind limb skeletal muscle from ischemia/reperfusion injury by alleviating oxidative stress and inflammatory responses via the MAPK/NF-휅B pathways.
Bilobalide may be a promising drug for I/R-injured muscle tissue. However, the specific mechanisms for the protective effects still need further study.
临床上，骨骼肌缺血/再灌注损伤是一种危及生命的综合征，常由骨骼肌损伤引起，其特征为氧化应激和炎症反应。已发现银杏内酯具有抗氧化和抗炎作用。然而，尚不清楚银杏内酯是否能保护骨骼肌免受缺血/再灌注损伤。
通过苏木精-伊红染色及评估肌肉组织的湿重/干重比，研究银杏内酯对缺血/再灌注损伤骨骼肌的影响。然后，我们测量了脂质过氧化、抗氧化活性和炎性细胞因子水平。此外，进行蛋白质印迹法检测MAPK/NF-κB信号通路成员的蛋白水平。
银杏内酯治疗可通过MAPK/NF-κB信号通路减轻氧化应激和炎症反应，从而保护后肢骨骼肌免受缺血/再灌注损伤。
银杏内酯可能是一种对缺血/再灌注损伤肌肉组织有前景的药物。然而，其保护作用的具体机制仍需进一步研究。
