Department of Preventive Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
National Clinical Research Center for Oral Diseases, Shanghai, China.
BMC Oral Health. 2020 Jul 9;20(1):196. doi: 10.1186/s12903-020-01188-2.
Herpesviruses and bacteria and their interplay have long been believed to play important roles in the pathogenesis of periodontitis, but other microbial entities in the oral environment might also be involved. Anelloviruses are commonly detected in human, including in oral samples. The aim of the present study was to explore the occurrence and co-occurrence of human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), and human anelloviruses (HTTVs) in gingival tissue samples collected from participants recruited in Shanghai, China.
Gingival tissues were collected from 159 participants (57 with aggressive periodontitis (AP), 59 with chronic periodontitis (CP) and 43 with healthy periodontal status). The presence of HCMV, EBV, torque teno virus (TTV), torque teno mini virus (TTMV) and torque teno midi virus (TTMDV) DNA was detected by nested-PCR. The virus loads were quantified by real-time PCR.
The detection rates of EBV, TTV, TTMV and TTMDV were significantly higher in the AP and CP groups compared to the healthy group (all P < 0.01). A statistically significant association was found between EBV, TTV and TTMV virus load and periodontitis (all P < 0.05). Participants infected with EBV showed significantly higher infection rates and higher virus loads of TTV and TTMV than the EBV-negative group (all P < 0.05). The coexistence rates of EBV and anelloviruses and the coexistence of three HTTVs were significantly higher in AP and CP groups (all P < 0.01).
Collectively, results obtained in this study suggest that HTTVs and the coexistence of EBV and HTTVs in particular, may be associated with periodontitis. Possible mechanisms of the interaction between herpesviruses and anelloviruses in the context of periodontitis require further investigation.
疱疹病毒和细菌及其相互作用长期以来一直被认为在牙周炎的发病机制中发挥重要作用，但口腔环境中的其他微生物实体也可能参与其中。细小病毒属病毒通常在人类中检测到，包括在口腔样本中。本研究旨在探索人巨细胞病毒（HCMV）、爱泼斯坦-巴尔病毒（EBV）和人细小病毒（HTTVs）在上海招募的参与者的牙龈组织样本中的发生和共同发生。
从 159 名参与者（57 名侵袭性牙周炎（AP）、59 名慢性牙周炎（CP）和 43 名健康牙周状况）中采集牙龈组织。通过巢式 PCR 检测 HCMV、EBV、转矩腾病毒（TTV）、转矩腾微小病毒（TTMV）和转矩腾中型病毒（TTMDV）DNA 的存在。通过实时 PCR 定量病毒载量。
AP 和 CP 组 EBV、TTV、TTMV 和 TTMDV 的检出率明显高于健康组（均 P < 0.01）。EBV、TTV 和 TTMV 病毒载量与牙周炎之间存在统计学显著关联（均 P < 0.05）。感染 EBV 的参与者的 TTV 和 TTMV 感染率和病毒载量明显高于 EBV 阴性组（均 P < 0.05）。AP 和 CP 组 EBV 和细小病毒属病毒的共存率以及三种 HTTVs 的共存率明显更高（均 P < 0.01）。
总之，本研究结果表明，HTTVs 特别是 EBV 和 HTTVs 的共存可能与牙周炎有关。疱疹病毒和细小病毒属病毒之间相互作用的可能机制需要进一步研究。
