Takahashi M, Harris J P
Division of Otolaryngology/Head and Neck Surgery, University of California, San Diego Medical Center.
Acta Otolaryngol. 1988 Nov-Dec;106(5-6):409-16. doi: 10.3109/00016488809122264.
The distribution and anatomic localization of immunocompetent cells in normal mouse endolymphatic sac (ES) were examined by immunohistochemical methods. Antibodies against T cells (anti-Thy-1, -Lyt-1, -Lyt-2), macrophage (anti-Mac-1) and immunoglobulins (anti-IgM, -IgG, -IgA) were employed in an indirect technique utilizing strept-avidin-biotin complex. In the normal mouse ES, each of these cells could be detected by a difference in its frequency and distribution within and around the ES. Thy-1 positive (Thy-1+) cells are the most predominant in this tissue and can be seen throughout the ES. Lyt-1+ cells were found within the ES epithelium and in the perisaccular region, whereas Lyt-2+ cells were rarely present. Mac-1+ cells were present primarily in the lumen of the distal portion of the ES. IgM-bearing (IgM+) cells were seen in the subepithelial region, IgG+ cells were occasionally detected in the lumen and only a few IgA+ cells were present in the perisaccular region. This study revealed that the ES has the necessary immunological components for antigen processing and the generation of local immune responses within the inner ear.
采用免疫组织化学方法检测正常小鼠内淋巴囊（ES）中免疫活性细胞的分布及解剖定位。利用链霉亲和素-生物素复合物的间接技术，使用抗T细胞抗体（抗Thy-1、-Lyt-1、-Lyt-2）、抗巨噬细胞抗体（抗Mac-1）和抗免疫球蛋白抗体（抗IgM、-IgG、-IgA）。在正常小鼠内淋巴囊中，这些细胞中的每一种都可以通过其在内淋巴囊内部及周围的频率和分布差异来检测。Thy-1阳性（Thy-1+）细胞在该组织中最为主要，且在内淋巴囊中均可见到。Lyt-1+细胞见于内淋巴囊上皮及囊周区域，而Lyt-2+细胞很少出现。Mac-1+细胞主要存在于内淋巴囊远端部分的管腔内。含IgM（IgM+）细胞见于上皮下区域，IgG+细胞偶尔在管腔内检测到，仅少数IgA+细胞存在于囊周区域。本研究表明，内淋巴囊具有在内耳内进行抗原处理及产生局部免疫反应所需的免疫成分。
