Angural Steffy, Rana Monika, Sharma Alisha, Warmoota Rahul, Puri Neena, Gupta Naveen
Department of Microbiology, BMS Block 1, South Campus, Panjab University, Chandigarh, 160014 India.
Department of Industrial Microbiology, Guru Nanak Khalsa College, Yamunanagar, 135001 Haryana India.
Indian J Microbiol. 2020 Sep;60(3):383-387. doi: 10.1007/s12088-020-00867-6. Epub 2020 Apr 20.
Microbial enzymes are the safe alternatives to chemical based bleaching of pulp in paper mills. For effective biobleaching, both hemicellulolytic and lignolytic enzymes are required. This study reports laccase (L) + xylanase (X) and laccase (L) + mannanase (M) enzyme concoctions for pulp biobleaching derived from sp. LX and sp. LM isolated from the decaying organic matter. All enzymes were thermo-alkali-stable, hence were suitable for their application in pulp biobleaching. When a mixture of L + X/L + M was used for mixedwood pulp biobleaching, 46.32/40.25% reduction in kappa number; 13.21/10.01% and 3.36/2.76% improvement in brightness and whiteness was achieved respectively. Moreover, no laccase mediator system was required in the current process. Significant changes in the structure of enzymatically treated pulp were also observed. All these properties make these concoctions of enzymes suitable for their application in pulp and paper mill.
微生物酶是造纸厂中基于化学方法的纸浆漂白的安全替代方案。为了实现有效的生物漂白，半纤维素分解酶和木质素分解酶都是必需的。本研究报道了从腐烂有机物中分离出的LX菌和LM菌产生的用于纸浆生物漂白的漆酶（L）+木聚糖酶（X）和漆酶（L）+甘露聚糖酶（M）酶混合物。所有酶都具有热碱稳定性，因此适合用于纸浆生物漂白。当使用L+X/L+M混合物进行混合木浆生物漂白时，卡伯值分别降低了46.32%/40.25%；亮度分别提高了13.21%/10.01%，白度分别提高了3.36%/2.76%。此外，当前工艺不需要漆酶介导体系。还观察到酶处理纸浆的结构有显著变化。所有这些特性使得这些酶混合物适合在制浆造纸厂中应用。
