Nayaka Ni Made D M W, Fidrianny Irda, Hartati Rika, Singgih Marlia
Pharmaceutical Biology Department, School of Pharmacy- Bandung Institute of Technology, Jl. Ganesa 10, Bandung, Indonesia.
J Taibah Univ Med Sci. 2020 May 14;15(3):211-217. doi: 10.1016/j.jtumed.2020.04.005. eCollection 2020 Jun.
Honey is an apiary product with various medicinal properties resulting from its bioactive compounds. Here, we aimed to determine the antioxidant and antibacterial properties of the Indonesian honey extracts and their correlation with total phenolic content (TPC) and total flavonoid content (TFC).
We extracted ethyl acetate-n-hexane and two types of ethanolic extracts from crude honey. Phenols and flavonoid content were calculated using spectroscopy. The antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric ion reducing antioxidant power (FRAP) assays, and it was reflected via the antioxidant activity index (AAI). An agar diffusion test was used to test the antimicrobial activity.
The ethyl acetate extract of the Karangasem honey provided the highest amount of phenolic and flavonoid content, and the strongest antioxidant activity using DPPH and FRAP assays. The ethanolic honey extracts were active against and ; in this regard, the strongest effect was noticed from the Singaraja honey extract. The positive significant correlations between TPC and AAI were observed in all samples. Similar results also appeared between phenolic and flavonoid compounds and their antibacterial activity in most of the tested samples.
In our study, honey extracts possessed antioxidant and antibacterial activities that were mostly related to the qualitative and quantitative properties of phenols and flavonoids. Geographical origin brought variations in the phytochemical profiles and bioactivities of honey.
蜂蜜是一种蜂产品，其生物活性化合物赋予了它多种药用特性。在此，我们旨在测定印度尼西亚蜂蜜提取物的抗氧化和抗菌特性，以及它们与总酚含量（TPC）和总黄酮含量（TFC）的相关性。
我们从粗蜂蜜中提取了乙酸乙酯 - 正己烷提取物和两种乙醇提取物。使用光谱法计算酚类和黄酮类化合物的含量。通过2,2 - 二苯基 - 1 - 苦基肼（DPPH）和铁离子还原抗氧化能力（FRAP）测定来评估抗氧化活性，并通过抗氧化活性指数（AAI）来反映。采用琼脂扩散试验检测抗菌活性。
卡朗阿森蜂蜜的乙酸乙酯提取物的酚类和黄酮类化合物含量最高，并且在DPPH和FRAP测定中表现出最强的抗氧化活性。蜂蜜乙醇提取物对[具体菌种1]和[具体菌种2]有活性；在这方面，从新加拉惹蜂蜜提取物中观察到最强的效果。在所有样品中均观察到TPC与AAI之间存在显著正相关。在大多数测试样品中，酚类和黄酮类化合物与其抗菌活性之间也出现了类似的结果。
在我们的研究中，蜂蜜提取物具有抗氧化和抗菌活性，这主要与酚类和黄酮类化合物的定性和定量特性有关。地理来源导致了蜂蜜植物化学特征和生物活性的差异。
