Yang Ying, Ying Gaoxiang, Wu Shanshan, Wu Fengtian, Chen Zhi
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Zhejiang, China.
Infect Agent Cancer. 2020 Jul 6;15:44. doi: 10.1186/s13027-020-00309-4. eCollection 2020.
Hepatitis B virus (HBV) causes hepatitis, which progresses to fatal liver diseases and remains a global health problem. Current treatments for chronic hepatitis B are unable to cure hepatitis. Thus, new antiviral drugs must be developed. In this study, the viral inhibition effects of dandelion and taraxasterol were assessed in HepG2.2.15 cell line. F.H.Wigg. (compositae) with English name dandelion is used as a traditional herb for liver disorders and as a common antiviral agent. Taraxasterol is one of the active compounds of dandelion. The secretion of HBV DNA and HBV surface antigen (HBsAg) and HBeAg was detected using fluorescence quantitative PCR (qPCR) and ELISA, respectively. Intracellular HBsAg was detected by immunofluorescence. In order to demonstrate the potential mechanism of anti-viral activity, the expression levels of host factors polypyrimidine tract binding protein 1 (PTBP1) and sirtuin 1 (SIRT1) were detected with Western blotting and qPCR. Dandelion and taraxasterol effectively reduced the secretion of HBsAg, HBeAg and the HBV DNA in cell supernatants, and significantly reduced the intracellular HBsAg as indicated by immunofluorescence results. Taraxasterol may be one of the main effective components of dandelion. It significantly decreased the protein expression levels of PTBP1 and SIRT1. The present study revealed that dandelion and its component taraxasterol could inhibit HBV and may be a potential anti-HBV drug, whose potential targets were the host factors PTBP1 and SIRT1.
乙型肝炎病毒（HBV）可引发肝炎，进而发展为致命的肝脏疾病，仍然是一个全球性的健康问题。目前针对慢性乙型肝炎的治疗方法无法治愈肝炎。因此，必须研发新的抗病毒药物。在本研究中，在HepG2.2.15细胞系中评估了蒲公英和蒲公英甾醇的病毒抑制作用。菊科植物蒲公英（F.H.Wigg.），英文名dandelion，作为一种传统草药用于治疗肝脏疾病，也是一种常见的抗病毒剂。蒲公英甾醇是蒲公英的活性成分之一。分别使用荧光定量PCR（qPCR）和酶联免疫吸附测定（ELISA）检测HBV DNA、乙肝表面抗原（HBsAg）和乙肝e抗原（HBeAg）的分泌情况。通过免疫荧光检测细胞内的HBsAg。为了阐明抗病毒活性的潜在机制，采用蛋白质免疫印迹法和qPCR检测宿主因子多聚嘧啶序列结合蛋白1（PTBP1）和沉默调节蛋白1（SIRT1）的表达水平。蒲公英和蒲公英甾醇有效降低了细胞上清液中HBsAg、HBeAg和HBV DNA的分泌，免疫荧光结果表明细胞内HBsAg也显著减少。蒲公英甾醇可能是蒲公英的主要有效成分之一。它显著降低了PTBP1和SIRT1的蛋白表达水平。本研究表明，蒲公英及其成分蒲公英甾醇可抑制HBV，可能是一种潜在的抗HBV药物，其潜在靶点为宿主因子PTBP1和SIRT1。
