Khan S A, Schmidt K, Hallin P, Di Pauli R, De Geyter C, Nieschlag E
Max Planck Clinical Research Unit for Reproductive Medicine, Institute of Reproductive Medicine of the University, Münster, F.R.G.
Mol Cell Endocrinol. 1988 Aug;58(2-3):221-30. doi: 10.1016/0303-7207(88)90158-x.
Human testicular cytosol and ovarian follicular fluid were analyzed for the presence of interleukin-1 (IL-1)-like factors. Both the follicular fluid and testis cytosol preparations exhibited significant IL-1-like activity as determined by the murine thymocyte proliferation bioassay. The dose-response lines obtained with the gonadal preparations were parallel to each other and to those obtained with monocyte-derived IL-1 and the activity of the gonadal IL-1 could be neutralized by specific IL-1 antibodies. After gel chromatography of human follicular fluid (hFF) and human testis cytosol (hTC) proteins, IL-1 activity was found in the molecular weight region between 30 and 50 kilodaltons (kDa). Chromatofocusing of IL-1 from hFF and hTC revealed that the major part of IL-1 in both cases exhibited similar charge properties (pI less than 6.0). However, two extra peaks (pI 7.0 and greater than 9.0, respectively) were observed in hFF preparations. After isoelectrofocusing (IEF), IL-1 activity of hFF was also found in two different pH regions; a broad area of activity was localized between pH 5.5 and 7.0, while a sharp peak was observed with an approximate pI value of 9.5. Re-chromatofocusing or IEF of alkaline IL-1-like activity resulted in a heterogeneous profile of IL-1-like activity suggesting that the alkaline material may represent either a precursor or an aggregated form of the acidic IL-1. None of the IL-1 peaks obtained from hFF or hTC exhibited IL-2 activity as assessed in a specific IL-2 bioassay. The results of the present study indicate that both gonads may produce high amounts of IL-1-like factor(s) which might play a regulatory role in normal gonadal function.
对人睾丸胞质溶胶和卵巢卵泡液进行了白细胞介素-1（IL-1）样因子的分析。通过小鼠胸腺细胞增殖生物测定法确定，卵泡液和睾丸胞质溶胶制剂均表现出显著的IL-1样活性。用性腺制剂获得的剂量反应线相互平行，且与单核细胞衍生的IL-1所获得的线平行，并且性腺IL-1的活性可被特异性IL-1抗体中和。对人卵泡液（hFF）和人睾丸胞质溶胶（hTC）蛋白进行凝胶色谱分析后，在分子量30至50千道尔顿（kDa）之间的区域发现了IL-1活性。对hFF和hTC中的IL-1进行色谱聚焦分析表明，两种情况下IL-1的主要部分都表现出相似的电荷特性（pI小于6.0）。然而，在hFF制剂中观察到两个额外的峰（分别为pI 7.0和大于9.0）。等电聚焦（IEF）后，hFF的IL-1活性也在两个不同的pH区域被发现；一个广泛的活性区域位于pH 5.5至7.0之间，而在近似pI值为9.5处观察到一个尖锐的峰。对碱性IL-1样活性进行再色谱聚焦或IEF后，得到了IL-1样活性的异质性图谱，这表明碱性物质可能代表酸性IL-1的前体或聚集形式。在特定的IL-2生物测定中评估，从hFF或hTC获得的IL-1峰均未表现出IL-2活性。本研究结果表明，两性性腺可能都产生大量的IL-1样因子，这些因子可能在正常性腺功能中发挥调节作用。
