宽叶香蒲中草酸预处理及酶糖化用于还原糖生产的优化
Optimization of oxalic acid pre-treatment and enzymatic saccharification in Typha latifolia for production of reducing sugar.
作者信息
Ramaiah Sunil Kodishetty, Thimappa Girisha Shringala, Nataraj Lokesh Kyathasandra, Dasgupta Proteek
机构信息
Bioenergy Lab, Department of Biotechnology, Bangalore University, Bengaluru, Karnataka, 560056, India.
Department of Biotechnology, M.S. Ramaiah Institute of Technology, Bengaluru, Karnataka, 560054, India.
出版信息
J Genet Eng Biotechnol. 2020 Jul 9;18(1):28. doi: 10.1186/s43141-020-00042-w.
BACKGROUND
Plants with high biomass can be manipulated for their reducing sugar content which ultimately upon fermentation produces ethanol. This concept was used to enhance the production of reducing sugar from cattail (Typha latifolia) by oxalic acid (OAA) pre-treatment followed by enzymatic saccharification.
RESULT
The optimum condition of total reducing sugar released from OAA pre-treatment was found to be 22.32 mg/ml (OAA-1.2%; substrate concentration (SC)-6%; reaction time (RT)-20 min) using one variable at a time (OVAT). Enzymatic saccharification yielded 45.21 mg/ml of reducing sugar (substrate concentration (SC)-2.4%; enzymatic dosage-50 IU/g; pH 7.0; temp-50 °C) using response surface methodology (RSM).
CONCLUSION
We conclude that Typha can be used as a potential substrate for large-scale biofuel production, employing economical bioprocessing strategies.
背景
具有高生物量的植物可通过调节其还原糖含量，最终经发酵生产乙醇。本研究利用这一概念，通过草酸（OAA）预处理，随后进行酶促糖化，提高香蒲（宽叶香蒲）还原糖的产量。
结果
采用一次一个变量法（OVAT），发现OAA预处理释放总还原糖的最佳条件为22.32毫克/毫升（OAA - 1.2%；底物浓度（SC） - 6%；反应时间（RT） - 20分钟）。使用响应面法（RSM）进行酶促糖化，得到还原糖产量为45.21毫克/毫升（底物浓度（SC） - 2.4%；酶用量 - 50国际单位/克；pH 7.0；温度 - 50°C）。
结论
我们得出结论，采用经济的生物加工策略，香蒲可作为大规模生物燃料生产的潜在底物。
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