Cázares-Camacho Rosario, Domínguez-Avila J Abraham, Astiazarán-García Humberto, Montiel-Herrera Marcelino, González-Aguilar Gustavo A
Coordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo A. C., Hermosillo, Mexico.
Cátedras CONACYT-Centro de Investigación en Alimentación y Desarrollo A. C., Hermosillo, Mexico.
J Sci Food Agric. 2021 Jan 30;101(2):497-504. doi: 10.1002/jsfa.10658. Epub 2020 Aug 4.
Oxidative stress has been implicated in the pathogenesis and progression of diabetes mellitus. Both can damage the brain. Mango and its by-products are sources of bioactive compounds with antioxidant properties. We hypothesized that mango cv. 'Ataulfo' peel and pulp mitigate oxidative stress in the brain of streptozotocin-induced diabetic rats.
Twenty-four male Wistar rats were divided into four groups: control, untreated diabetic (UD), diabetic treated with a mango-supplemented diet (MTD), and diabetic pretreated with a mango-supplemented diet (MPD). The rats were fed the different diets for 4 weeks after diabetes induction (MTD), or 2 weeks before and 4 weeks after induction (MPD). After the intervention, serum and brain (cerebellum and cortex) were collected to evaluate gene expression, enzyme activity, and redox biomarkers. Superoxide dismutase 2 (SOD2) expression increased in the cortex of the MTD group, whereas glutathione-S-transferase p1 (GSTp1) expression was higher in the cortex of the MTD group, and cortex and cerebellum of the MPD group. SOD1 activity was higher in the cerebellum and cortex of all diabetic groups, whereas GST activity increased in the cerebellum and cortex of the MPD group. Lipid peroxidation increased in the cerebellum and cortex of the UD group; however, a mango-supplemented diet prevented this increase in both regions, while also mitigating polyphagia and weight loss, and maintaining stable glycemia in diabetic rats.
We propose that mango exerts potent neuroprotective properties against diabetes-induced oxidative stress. It can be an alternative to prevent and treat biochemical alterations caused by diabetes. © 2020 Society of Chemical Industry.
氧化应激与糖尿病的发病机制及进展有关。二者均可损害大脑。芒果及其副产品是具有抗氧化特性的生物活性化合物的来源。我们推测芒果品种‘阿图尔福’的果皮和果肉可减轻链脲佐菌素诱导的糖尿病大鼠大脑中的氧化应激。
将24只雄性Wistar大鼠分为四组：对照组、未治疗的糖尿病组（UD）、用添加芒果的饮食治疗的糖尿病组（MTD）和用添加芒果的饮食预处理的糖尿病组（MPD）。糖尿病诱导后（MTD），大鼠接受不同饮食4周；或诱导前2周及诱导后4周（MPD）。干预后，收集血清和大脑（小脑和皮层）以评估基因表达、酶活性和氧化还原生物标志物。超氧化物歧化酶2（SOD2）表达在MTD组皮层中增加，而谷胱甘肽-S-转移酶p1（GSTp1）表达在MTD组皮层以及MPD组皮层和小脑中更高。所有糖尿病组的小脑和皮层中SOD1活性均较高，而MPD组的小脑和皮层中GST活性增加。UD组的小脑和皮层中脂质过氧化增加；然而，添加芒果的饮食可防止这两个区域的这种增加，并减轻多食和体重减轻，同时使糖尿病大鼠的血糖保持稳定。
我们提出芒果对糖尿病诱导的氧化应激具有强大的神经保护作用。它可以作为预防和治疗糖尿病引起的生化改变的一种替代方法。©2020化学工业协会。
