Abdou Heba M, Hamaad Fatma A, Abd Elmageed Ghada M, Ghoneum Mamdooh H
Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt.
Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, Egypt.
Evid Based Complement Alternat Med. 2023 Jul 19;2023:8248576. doi: 10.1155/2023/8248576. eCollection 2023.
Type 2 diabetes mellitus (T2DM) is a common metabolic disease accompanied by cognitive impairment, hippocampal malfunctioning, and inflammation. Biobran/MGN-3, an arabinoxylan rice bran, has been shown to have an antidiabetic effect in streptozotocin-induced diabetic rats. The present study investigates Biobran's effect against diabetes-induced cognitive impairment and synaptotoxicity in the hippocampus via oxidative stress and the IR/A/NF-B signaling pathway in rats. Diabetes was induced via i.p. injection of streptozotocin (STZ) (40 mg/kg BW); STZ-treated rats were then administered Biobran (100 mg/kg BW) for 4 wks. Biobran supplementation improved motor coordination and muscular strength, as assessed by Kondziella's inverted screen test. Biobran also improved concentration levels of glutathione (GSH), antioxidant enzymes, acetylcholine (ACh), dopamine, serotonin, insulin receptor (IR), and alpha serine-threonine protein kinase (Akt); it protected against elevated levels of glucose, total cholesterol, triglycerides, oxidative stress markers, TBARS, NO, AChE, and MAO; and it significantly decreased inflammatory cytokines levels of IL-1, NF-B, TNF-, and amyloid . Moreover, Biobran ameliorated hippocampal histological alterations. Immunohistochemical observations showed that Biobran reduced overexpression of hippocampal synaptophysin and Ki67 relative to untreated diabetic rats. Biobran may ameliorate hippocampal alterations in diabetic rats via its antidiabetic, antiproliferative, anti-inflammatory, antiapoptotic, and antioxidant effects.
2型糖尿病（T2DM）是一种常见的代谢性疾病，伴有认知障碍、海马功能失调和炎症。生物麸/ MGN - 3，一种阿拉伯木聚糖米糠，已被证明在链脲佐菌素诱导的糖尿病大鼠中具有抗糖尿病作用。本研究通过氧化应激以及大鼠体内的胰岛素受体/ A/核因子κB信号通路，探讨生物麸对糖尿病诱导的认知障碍和海马突触毒性的影响。通过腹腔注射链脲佐菌素（STZ）（40mg/kg体重）诱导糖尿病；然后给STZ处理的大鼠施用生物麸（100mg/kg体重），持续4周。通过孔齐埃拉倒屏试验评估，补充生物麸改善了运动协调性和肌肉力量。生物麸还提高了谷胱甘肽（GSH）、抗氧化酶、乙酰胆碱（ACh）、多巴胺、血清素、胰岛素受体（IR）和α丝氨酸 - 苏氨酸蛋白激酶（Akt）的浓度水平；它预防了葡萄糖、总胆固醇、甘油三酯、氧化应激标志物、硫代巴比妥酸反应物（TBARS）、一氧化氮（NO）、乙酰胆碱酯酶（AChE）和单胺氧化酶（MAO）水平的升高；并且它显著降低了白细胞介素 - 1、核因子κB、肿瘤坏死因子 - 和淀粉样蛋白等炎症细胞因子的水平。此外，生物麸改善了海马的组织学改变。免疫组织化学观察表明，相对于未治疗的糖尿病大鼠，生物麸减少了海马突触素和Ki67的过表达。生物麸可能通过其抗糖尿病、抗增殖、抗炎、抗凋亡和抗氧化作用改善糖尿病大鼠的海马改变。
