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大鼠耳蜗和前庭毛细胞中钙结合蛋白的免疫细胞化学检测

Immunocytochemical detection of calcium-binding protein in the cochlear and vestibular hair cells of the rat.

作者信息

Rabié A, Thomasset M, Legrand C

出版信息

Cell Tissue Res. 1983;232(3):691-6. doi: 10.1007/BF00216440.

DOI:10.1007/BF00216440
PMID:6349822
Abstract

Specific antibodies raised against human cerebellar calcium-binding protein (CaBP) intensely labelled the cochlear hair cells of the rat. The vestibular hair cells also stained weakly. In both inner and outer cochlear hair cells, the cuticular plate was the most stained area. These results suggest that CaBP may prevent excessive concentrations of intracellular calcium and thus modulate some Ca2+-mediated biochemical processes, especially at the level of the cuticular plate and stereocilia; CaBP could be involved in the mechanochemical coupling of hearing or vestibular function.

摘要

针对人类小脑钙结合蛋白(CaBP)产生的特异性抗体强烈标记了大鼠的耳蜗毛细胞。前庭毛细胞也有弱染色。在耳蜗内、外毛细胞中,角质板是染色最深的区域。这些结果表明,CaBP可能防止细胞内钙浓度过高,从而调节一些Ca2+介导的生化过程,特别是在角质板和静纤毛水平;CaBP可能参与听觉或前庭功能的机械化学偶联。

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Immunocytochemical detection of 28 000-MW calcium-binding protein in horizontal cells of the rat retina.大鼠视网膜水平细胞中28000道尔顿钙结合蛋白的免疫细胞化学检测
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