Center of Stomatology, Xiangya Hospital, Institute of Oral Precancerous Lesions, Central South University, Changsha, China.
GeneTalks Biotech Co., Ltd., Changsha, China.
Oral Dis. 2021 Mar;27(2):226-239. doi: 10.1111/odi.13545. Epub 2020 Aug 6.
Oral cancer is among most common neoplasm of oral cavity; in many cases, it develops at the site of premalignant lesion. Areca nut has been identified as a carcinogen, which was proved to promote the inflammation level and contributes to oral malignancy. Chewing areca nut is the main cause of the premalignant disease oral submucous fibrosis (OSF). Bacterial alterations were suggested to be assonated with oral cancer progression. Therefore, the present study was carried out to determine the changes of microbiota in the mucosa along stage of development of oral cancer with areca nut chewing. 162 participants, reporting to department of oral medical center, were enrolled into the study which includes 45 patients each of OSF, 42 of oral cancer, 29 healthy controls (HC) with areca nut chewing, and 46 healthy controls (HC) never chewing areca nut. Oral swabbing of tongue dorsum, buccal mucosa, and gingiva was evaluated by MiSeq platform of the V3-V4 region of the 16S rRNA gene. These data revealed microbial changes that may mirror oral cancer progression and reflect clinical preconditions such as areca nut chewing. Consequently, revealing microbial changes in patients with oral squamous cell carcinomas and the premalignant disease oral submucous fibrosis (OSF) with areca nut chewing might improve our understanding of the pathobiology of the disease and help in the design of novel diagnostic and treatment strategies.
口腔癌是最常见的口腔恶性肿瘤之一；在许多情况下，它发生在癌前病变部位。槟榔已被确定为一种致癌物质，它被证明可促进炎症水平并导致口腔恶性肿瘤。咀嚼槟榔是导致癌前疾病口腔黏膜下纤维性变（OSF）的主要原因。细菌变化被认为与口腔癌的进展有关。因此，本研究旨在确定在咀嚼槟榔的情况下，口腔癌发展过程中黏膜微生物群的变化。共有 162 名参与者向口腔医学中心报告，被纳入本研究，其中包括 45 名 OSF 患者、42 名口腔癌患者、29 名有咀嚼槟榔习惯的健康对照组（HC）和 46 名从未咀嚼槟榔的健康对照组（HC）。通过 MiSeq 平台对舌背、颊黏膜和牙龈的 16S rRNA 基因的 V3-V4 区进行了口腔拭子检测。这些数据揭示了可能反映口腔癌进展的微生物变化，并反映了槟榔咀嚼等临床前期情况。因此，揭示咀嚼槟榔的口腔鳞状细胞癌和癌前疾病口腔黏膜下纤维性变（OSF）患者的微生物变化，可能有助于我们了解疾病的病理生物学，并有助于设计新的诊断和治疗策略。
